Evolutionary expansion and functional diversification of oligopeptide transporter gene family in rice.
Evolutionary expansion and functional diversification of oligopeptide transporter gene family in rice.
复制标题
水稻寡肽转运蛋白基因家族的进化扩展和功能多样化
DOI：
10.1186/1939-8433-5-12
复制
发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Liu T;Zeng J;Xia K;Fan T;Li Y;Wang Y;Xu X;Zhang M
研究背景寡肽转运蛋白（oligopeptide transporters，OPTs）在有机氮化合物的运输中起重要作用，通常与蛋白质快速水解的组织如萌发的种子和衰老的叶片相关联。本研究对水稻中的16个水稻OPT基因进行了研究。在水稻基因组中鉴定出10个基因片段，并将其分为6个片段，分别归属于PT和YSL两个亚科。在水稻基因组进化过程中，16个基因进化扩展的主要机制包括片段重复和串联重复。重复事件发生时间的计算表明，16个基因起源于9个原始OsOPTs基因，重复事件可分为3个进化阶段。第一个进化阶段发生在大约5000万年前（Mya），涉及四个新基因的进化。第二个进化阶段大约是20 Mya，并以两个新基因的出现为标志，第三个进化阶段大约是9 Mya，两个新基因进化。对表达数据库的挖掘和RT-PCR分析表明，多数重复OsOPTs基因的表达具有较高的组织特异性。使用半定量RT-PCR和MPSS数据评估16个基因的不同表达模式。OsOPTs基因的表达水平受非生物胁迫和生物胁迫的调控，表明这些基因产物可能参与了水稻的逆境适应。5个水稻特异性OsOST基因突变体均表现出异常的生长发育，初步分析表明它们可能是水稻生长发育所必需的。
BackgroundOligopeptide transporters (OPTs) play important roles in the mobilization of organic nitrogenous compounds and usually associate with tissues that show signs of rapid protein hydrolysis, such as germinating seeds and senescing leaves. This study is to investigate riceOPTgenes.ResultsA total of sixteenOsOPTgenes (OsforOryza sativeL.) were identified in the rice genome, which were then classified into six sections that belong to two subfamilies (the PT and YSL subfamily). The major mechanisms for evolutionary expansion of the sixteen genes during the rice genome evolution include segmental and tandem duplication. Calculation of the duplication event dates indicated that the sixteen genes originated from nine originalOsOPTgenes, and the duplication events could be classified into three evolutionary stages. The first evolutionary stage occurred approximately 50 million years ago (Mya) and involved the evolution of four new genes. The second evolutionary stage was approximately 20 Mya and was marked by the appearance of two new genes, and the third evolutionary stage was approximately 9 Mya when two new genes evolved. Mining of the expression database and RT-PCR analysis indicated that the expression of most duplicatedOsOPTgenes showed high tissue specificities. Diverse expression patterns for the sixteen genes were evaluated using both semi-quantitative RT-PCR and the MPSS data. Expression levels of someOsOPTgenes were regulated by abiotic and biotic stresses suggesting the potential involvement of these gene products in rice stress adaptation. FiveOsOPTgene mutants showed abnormal development and growth, the primary analysis of fiveOsOPTgene mutants suggested that they may be necessary for rice development.ConclusionsThese results suggested that rice-specificOsOPTgenes might be potentially useful in improving rice.
登录
查看更多内容
DOI：
10.1073/pnas.93.15.7783
发表时间：
1996-07-23
影响因子：
11.1
作者：
Hirochika, H;Sugimoto, K;Kanda, M
通讯作者：
Kanda, M
影响因子：
7.3
作者：
Bogs, J;Bourbouloux, A;Delrot, S
通讯作者：
Delrot, S
DOI：
10.1007/0-387-25856-6_3
发表时间：
2006-01-01
期刊：
Genetic engineering
影响因子：
--
作者：
Lubkowitz, Mark
通讯作者：
Lubkowitz, Mark
影响因子：
3.6
作者：
Lubkowitz, MA;Barnes, D;Becker, JM
通讯作者：
Becker, JM
影响因子：
3
作者：
Guo, YL;Ge, S
通讯作者：
Ge, S