NMR structure of a vestigial nuclease provides insight into the evolution of functional transitions in viral dsDNA packaging motors.
NMR structure of a vestigial nuclease provides insight into the evolution of functional transitions in viral dsDNA packaging motors.
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DOI：
10.1093/nar/gkaa874
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发表时间：
2020-11-18
影响因子：
14.9
通讯作者：
Morais MC
中科院分区：
文献类型：
--
作者：
Mahler BP;Bujalowski PJ;Mao H;Dill EA;Jardine PJ;Choi KH;Morais MC
Double-stranded DNA viruses use ATP-powered molecular motors to package their genomic DNA. To ensure efficient genome encapsidation, these motors regulate functional transitions between initiation, translocation, and termination modes. Here, we report structural and biophysical analyses of the C-terminal domain of the bacteriophage phi29 ATPase (CTD) that suggest a structural basis for these functional transitions. Sedimentation experiments show that the inter-domain linker in the full-length protein promotes oligomerization and thus may play a role in assembly of the functional motor. The NMR solution structure of the CTD indicates it is a vestigial nuclease domain that likely evolved from conserved nuclease domains in phage terminases. Despite the loss of nuclease activity, fluorescence binding assays confirm the CTD retains its DNA binding capabilities and fitting the CTD into cryoEM density of the phi29 motor shows that the CTD directly binds DNA. However, the interacting residues differ from those identified by NMR titration in solution, suggesting that packaging motors undergo conformational changes to transition between initiation, translocation, and termination. Taken together, these results provide insight into the evolution of functional transitions in viral dsDNA packaging motors.
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影响因子：
5.4
作者：
Cao, Sheng;Saha, Mitul;Morais, Marc C.
通讯作者：
Morais, Marc C.
影响因子：
3.7
作者：
Dixit, Aparna Banerjee;Ray, Krishanu;Black, Lindsay W.
通讯作者：
Black, Lindsay W.
影响因子：
14.9
作者：
Górecka KM;Komorowska W;Nowotny M
通讯作者：
Nowotny M
影响因子：
56.9
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GUO, PX;ERICKSON, S;ANDERSON, D
通讯作者：
ANDERSON, D
影响因子：
64.8
作者：
FINER, JT;SIMMONS, RM;SPUDICH, JA
通讯作者：
SPUDICH, JA