The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation.
The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation.
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大蒜组成的二烯基三硫化物通过SKN-1激活增加了秀丽隐杆线虫的寿命。
DOI：
10.1016/j.exger.2011.01.005
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发表时间：
2011-06
影响因子：
3.9
通讯作者：
Fisher AL
中科院分区：
文献类型：
--
作者：
Powolny AA;Singh SV;Melov S;Hubbard A;Fisher AL
葱属蔬菜的药用价值，如大蒜，在整个历史记录中已经注意到，包括预防癌症和心血管疾病。我们现在证明大蒜成分二烯丙基三硫化物（DATS）增加了C。elegans通过影响skn-1途径。用5-10 μM DATS处理蠕虫增加了蠕虫的平均寿命，即使在成年初期开始处理。为了探索DATS介导的寿命增加的机制，我们处理了daf-2，daf-16和eat-2突变体，发现DATS增加了daf-2和daf-16突变体的寿命，但没有eat-2突变体。微阵列实验表明，氧化应激和skn-1转录因子调控的一些基因也改变了DATS治疗。一致地，DATS处理导致skn-1靶基因gst-4的诱导，并且该诱导依赖于skn-1。我们还发现DATS对蠕虫寿命的影响取决于肠道和ASI神经元中的skn-1活性。总之，我们的数据表明，DATS能够通过增强促长寿转录因子skn-1的功能来增加蠕虫寿命。
Medicinal benefits of Allium vegetables, such as garlic, have been noted throughout recorded history, including protection against cancer and cardiovascular disease. We now demonstrate that garlic constituent diallyl trisulfide (DATS) increases longevity of C. elegans by affecting the skn-1 pathway. Treatment of worms with 5-10 μM DATS increased worm mean lifespan even when treatment is started during young adulthood. To explore the mechanisms involved in the DATS-mediated increase in longevity, we treated daf-2, daf-16, and eat-2 mutants and found that DATS increased the lifespan of daf-2 and daf-16 mutants, but not the eat-2 mutants. Microarray experiments demonstrated that a number of genes regulated by oxidative stress and the skn-1 transcription factor were also changed by DATS treatment. Consistently, DATS treatment leads to the induction of the skn-1 target gene gst-4, and this induction was dependent on skn-1. We also found that the effects of DATS on worm lifespan depend on skn-1 activity in both in the intestine and ASI neurons. Together our data suggest that DATS is able to increase worm lifespan by enhancing the function of the pro-longevity transcription factor skn-1.
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影响因子：
11.8
作者：
Gerisch, B;Weitzel, C;Antebi, A
通讯作者：
Antebi, A
影响因子：
7.8
作者：
Fisher, AL;Lithgow, GJ
通讯作者：
Lithgow, GJ
DOI：
10.1111/j.1349-7006.1999.tb00791.x
发表时间：
1999-06
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Gao CM;Takezaki T;Ding JH;Li MS;Tajima K
通讯作者：
Tajima K
影响因子：
7.4
作者：
Chen, C;Pung, D;Kong, ANT
通讯作者：
Kong, ANT
影响因子：
4.8
作者：
Fisher, Alfred L.;Page, Kathryn E.;Nash, Lindsey
通讯作者：
Nash, Lindsey