Human retinoblastoma susceptibility gene.-猫眼课题宝

人类视网膜母细胞瘤易感基因。

DOI：

发表时间：

1990

期刊：

Genetic engineering

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

C. C. Lai;W. Lee

很明显，在恢复RB基因表达后，裸鼠的RB缺陷肿瘤细胞失去了致瘤性。然而，RB基因抑制肿瘤的机制尚不清楚。为了回答这个问题，有必要对RB蛋白pp110RB的生物学活性进行更多的研究。最近的研究表明，几种致癌病毒蛋白，如SV40大T抗原（47）和腺病毒E1A蛋白（48），与RB蛋白结合。这些绑定的意义尚不清楚；然而，他们认为，病毒蛋白耗尽功能性RB蛋白可能提供了RB失活的另一种机制。继续研究自然发生的和工程的RB突变体可能会给我们提供一些关于RB蛋白的生物活性及其在肿瘤发生、分化、发育和基因调控中的作用的信息。此外，直接检测RB基因突变将有很大的临床应用价值。RB基因及基因产物的检测将有助于肿瘤易感性家庭的遗传诊断。因此，针对RB蛋白的抗体将成为临床医学诊断和/或预后应用的优秀工具。

It is clear that the RB-deficient tumor cells lost their tumorigenicity in nude mice after regaining the RB gene expression. However, the mechanism of tumor suppression by the RB gene is still unknown. More studies on the biological activities of RB protein, pp110RB, are necessary to answer this question. Recent studies have shown that several oncogenic viral proteins, such as SV40 large T antigen (47) and adenoviral E1A protein (48), bind to RB protein. The significance of these bindings remains unclear; nevertheless, they suggest that depletion of functional RB protein by viral proteins may provide another mechanism of RB inactivation. Continued study of naturally occurring as well as engineered RB mutants may give us some information on the biological activity of RB protein, and its roles in oncogenesis, differentiation, development and gene regulation. Additionally, direct detection of RB gene mutations would have great clinical utility. Probes for the RB gene and gene product will be useful for genetic diagnosis of cancer susceptibility in affected families. Therefore, antibodies to the RB protein will be excellent tools for diagnostic and/or prognostic application in clinical medicine.