Cadherin-11 controls otolith assembly: evidence for extracellular cadherin activity.
Cadherin-11 controls otolith assembly: evidence for extracellular cadherin activity.
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DOI：
10.1002/dvdy.22015
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发表时间：
2009-08
影响因子：
2.5
通讯作者：
Marrs, James A.
中科院分区：
文献类型：
--
作者：
Clendenon, Sherry G.;Shah, Bijal;Miller, Caroline A.;Schmeisser, Glen;Walter, Amanda;Gattone, Vincent H., II;Barald, Kate F.;Liu, Qin;Marrs, James A.
Cadherin-11/Cdh 11通过早期发育表达，并在内耳发育（耳基板和囊泡）期间强烈表达。在这里，我们表明，反义敲低Cdh 11在早期斑马鱼发育干扰耳石的形成。免疫荧光标记显示Cdh 11的表达集中在耳石上和耳石内。Cdh 11在耳上皮细胞的侧面（细胞-细胞接触的部位）和细胞质中微弱地检测到。强标记Cdh 11含有斑点内检测到耳淋巴（耳泡内的液体），并与耳石表面。BODIPY-神经胺标记的泡状结构中检测到的耳淋巴中的Cdh 11敲除胚胎更大，数量更多。我们提出的证据支持一个工作模型，泡状结构含有Cdh 11（可能含有生物矿化成分）出口到耳淋巴从耳上皮，坚持彼此和表面的耳石生长，促进耳石生长。
Cadherin-11/Cdh11 is expressed through early development and strongly during inner ear development (otic placode and vesicle). Here we show that antisense knockdown of Cdh11 during early zebrafish development interferes with otolith formation. Immunofluorescence labeling showed that Cdh11 expression was concentrated on and within the otolith. Cdh11 was faintly detected at the lateral surface (sites of cell-cell contact) of otic epithelial cells and in the cytoplasm. Strongly labeled Cdh11 containing puncta were detected within the otolymph (the fluid within the otic vesicle) and associated with the otolith surface. BODIPY-ceramine labeled vesicular structures detected in the otolymph were larger and more numerous in Cdh11 knockdown embryos. We present evidence supporting a working model that vesicular structures containing Cdh11 (perhaps containing biomineralization components) are exported from the otic epithelium into the otolymph, adhere to one another and to the surface of the growing otolith, facilitating otolith growth.
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影响因子：
1.5
作者：
Draper, BW;Morcos, PA;Kimmel, CB
通讯作者：
Kimmel, CB
影响因子：
2.7
作者：
Blasiole, Brian;Canfield, Victor A.;Levenson, Robert
通讯作者：
Levenson, Robert
DOI：
10.1016/j.asr.2003.09.043
发表时间：
2004-01-01
期刊：
SPACE LIFE SCIENCES: SEARCH FOR SIGNATURES OF LIFE, AND SPACE FLIGHT ENVIRONMENTAL EFFECTS ON THE NERVOUS SYSTEM
影响因子：
--
作者：
Ibsch, M;Anken, RH;Rahmann, H
通讯作者：
Rahmann, H
DOI：
10.1152/ajpcell.2002.282.1.c213
发表时间：
2002-01-01
影响因子：
5.5
作者：
Clendenon, JL;Phillips, CL;Dunn, KW
通讯作者：
Dunn, KW
影响因子：
14.9
作者：
OXTOBY, E;JOWETT, T
通讯作者：
JOWETT, T