RNA polymerase V targets transcriptional silencing components to promoters of protein-coding genes.
RNA polymerase V targets transcriptional silencing components to promoters of protein-coding genes.
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DOI：
10.1111/tpj.12034
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发表时间：
2013-01
期刊：
影响因子：
--
通讯作者：
Wierzbicki AT
中科院分区：
文献类型：
--
作者：
Zheng Q;Rowley MJ;Böhmdorfer G;Sandhu D;Gregory BD;Wierzbicki AT
转录基因沉默通过RNA指导的DNA甲基化（RdDM）和异染色质形成来控制转座子和其他重复元件。Arabidopsis RdDM途径的一个关键组分是ARGONAUTE 4（AGO 4），其与siRNA结合以介导DNA甲基化。在这里，我们表明，AGO 4优先目标嵌入在蛋白质编码基因的启动子内的转座因子。AGO 4的这种结合模式不能简单地用AGO 4结合的SiRNA的序列来解释;相反，AGO 4与特定基因启动子的结合也是由RNA聚合酶V产生的长链非编码RNA（lncRNA）介导的。lncRNA介导的AGO 4与基因启动子的结合将不对称DNA甲基化引导到这些基因组区域，并参与调节靶基因的表达。最后，AGO 4结合重叠的DNA甲基化位点的生物应激反应的影响。基于这些发现，我们提出AGO 4-定向RdDM的目标是负责在特定环境条件下控制基因表达的调控单位。
Transcriptional gene silencing controls transposons and other repetitive elements through RNA-directed DNA methylation (RdDM) and heterochromatin formation. A key component of the Arabidopsis RdDM pathway is ARGONAUTE4 (AGO4), which associates with siRNAs to mediate DNA methylation. Here, we show that AGO4 preferentially targets transposable elements embedded within promoters of protein-coding genes. This pattern of AGO4 binding cannot be simply explained by the sequences of AGO4-bound siRNAs; instead, AGO4 binding to specific gene promoters is also mediated by long non-coding RNAs (lncRNAs) produced by RNA polymerase V. lncRNA-mediated AGO4 binding to gene promoters directs asymmetric DNA methylation to these genomic regions and is involved in regulating the expression of targeted genes. Finally, AGO4 binding overlaps sites of DNA methylation affected by the biotic stress response. Based on these findings, we propose that the targets of AGO4-directed RdDM are regulatory units responsible for controlling gene expression under specific environmental conditions.
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DOI：
10.1101/sqb.1956.021.01.017
发表时间：
1956-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
MCCLINTOCK, B
通讯作者：
MCCLINTOCK, B
影响因子：
64.8
作者：
Cernilogar, Filippo M.;Onorati, Maria Cristina;Kothe, Greg O.;Burroughs, A. Maxwell;Parsi, Krishna Mohan;Breiling, Achim;Lo Sardo, Federica;Saxena, Alka;Miyoshi, Keita;Siomi, Haruhiko;Siomi, Mikiko C.;Carninci, Piero;Gilmour, David S.;Corona, Davide F. V.;Orlando, Valerio
通讯作者：
Orlando, Valerio
影响因子：
64.5
作者：
Li, Carey Fei;Pontes, Olga;Jacobsen, Steven E.
通讯作者：
Jacobsen, Steven E.
影响因子：
56.9
作者：
Erhard, Karl F., Jr.;Stonaker, Jennifer L.;Hollick, Jay B.
通讯作者：
Hollick, Jay B.
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.