Biosilica formation in spicules of the sponge Suberites domuncula: synchronous expression of a gene cluster.

Biosilica formation in spicules of the sponge Suberites domuncula: synchronous expression of a gene cluster.
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海绵 Suberites domunula 针状体中生物硅的形成:基因簇的同步表达

DOI:
10.1016/j.ygeno.2005.02.005
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发表时间:
2005
期刊:
影响因子:
4.4
通讯作者:
Müller WEG
Müller WEG
中科院分区:
生物学3区
文献类型:
--
作者:
Schröder HC;Perović-Ottstadt S;Grebenjuk VA;Engel S;Müller IM;Müller WEG

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海绵骨针的形成是一个复杂的形态发生过程。的原态系统被用来证明,在demosponge Suberites domuncula的合成的硅质骨针开始胞内,是依赖于浓度的柠檬酸。为了理解骨针的形成,分离出一组基因。在这个簇的中心是silicatein基因,它编码合成针状体的酶。该基因的一侧是锚蛋白重复基因,另一侧是肿瘤坏死因子受体相关因子和蛋白激酶基因。所有基因在暴露于谷氨酸后在原型体和完整动物中强烈表达,并且这种表达仅限于骨针形成开始或骨针在动物中维持的那些区域。我们的观察表明,在S. domuncula一个协调表达的物理连接的基因是必不可少的合成的主要骨骼元素。
The formation of spicules is a complicated morphogenetic process in sponges (phylum Porifera). The primmorph system was used to demonstrate that in the demosponge Suberites domuncula the synthesis of the siliceous spicules starts intracellularly and is dependent on the concentration of silicic acid. To understand spicule formation, a cluster of genes was isolated. In the center of this cluster is the silicatein gene, which codes for the enzyme that synthesizes spicules. This gene is flanked by an ankyrin repeat gene at one side and by a tumor necrosis factor receptor-associated factor and a protein kinase gene at the other side. All genes are strongly expressed in primmorphs and intact animals after exposure to silicic acid, and this expression is restricted to those areas where the spicule formation starts or where spicules are maintained in the animals. Our observations suggest that in S. domuncula a coordinated expression of physically linked genes is essential for the synthesis of the major skeletal elements.
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