Microchannel acoustophoresis does not impact survival or function of microglia, leukocytes or tumor cells.
Microchannel acoustophoresis does not impact survival or function of microglia, leukocytes or tumor cells.
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DOI：
10.1371/journal.pone.0064233
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Deierborg T
中科院分区：
文献类型：
--
作者：
Burguillos MA;Magnusson C;Nordin M;Lenshof A;Augustsson P;Hansson MJ;Elmér E;Lilja H;Brundin P;Laurell T;Deierborg T
使用声力以微流体规模操纵颗粒或细胞（即声泳）能够基于固有的细胞性质（例如尺寸、密度和可压缩性）进行非接触、无标记的分离。声电泳作为一种细胞分离技术在许多研究和临床领域具有很大的前景。然而，已经提出，作用于经历声电泳的细胞的力可以通过细微的表型变化影响细胞活力、增殖或细胞功能。如果是这种情况，这将表明声电泳方法对于许多细胞分析应用以及细胞治疗来说将是一种不太有用的工具。我们调查，第一次，几个关键方面的细胞变化后，声泳处理。我们使用了两种超声致动设置，一种用于细胞分选（10 Vpp工作电压），另一种接近系统可以产生的最大值（20 Vpp）。我们使用小胶质细胞并评估细胞活力和增殖，以及指示细胞表型中更微妙变化的炎症反应。此外，我们采用了类似的方法来监测人类前列腺癌细胞对声泳处理的反应。最后，我们分析了人类白细胞和血小板的呼吸特性，以探讨声泳处理是否有不利影响。BV2小胶质细胞在声泳处理后未发生改变，如通过凋亡和细胞周转测定以及炎症细胞因子反应在声泳后48小时所测量的。同样，我们发现声泳处理既不影响前列腺癌细胞的细胞活力，也不改变其前列腺特异性抗原分泌后雄激素受体激活。最后，人类血小板和白细胞显示声泳处理后线粒体呼吸功能和完整性不变。我们的结论是，微通道声电泳可用于有效的连续流动为基础的细胞分离，而不影响细胞活力，增殖，线粒体呼吸或炎症状态。
The use of acoustic forces to manipulate particles or cells at the microfluidic scale (i.e. acoustophoresis), enables non-contact, label-free separation based on intrinsic cell properties such as size, density and compressibility. Acoustophoresis holds great promise as a cell separation technique in several research and clinical areas. However, it has been suggested that the force acting upon cells undergoing acoustophoresis may impact cell viability, proliferation or cell function via subtle phenotypic changes. If this were the case, it would suggest that the acoustophoresis method would be a less useful tool for many cell analysis applications as well as for cell therapy. We investigate, for the first time, several key aspects of cellular changes following acoustophoretic processing. We used two settings of ultrasonic actuation, one that is used for cell sorting (10 Vpp operating voltage) and one that is close to the maximum of what the system can generate (20 Vpp). We used microglial cells and assessed cell viability and proliferation, as well as the inflammatory response that is indicative of more subtle changes in cellular phenotype. Furthermore, we adapted a similar methodology to monitor the response of human prostate cancer cells to acoustophoretic processing. Lastly, we analyzed the respiratory properties of human leukocytes and thrombocytes to explore if acoustophoretic processing has adverse effects. BV2 microglia were unaltered after acoustophoretic processing as measured by apoptosis and cell turnover assays as well as inflammatory cytokine response up to 48 h following acoustophoresis. Similarly, we found that acoustophoretic processing neither affected the cell viability of prostate cancer cells nor altered their prostate-specific antigen secretion following androgen receptor activation. Finally, human thrombocytes and leukocytes displayed unaltered mitochondrial respiratory function and integrity after acoustophoretic processing. We conclude that microchannel acoustophoresis can be used for effective continuous flow-based cell separation without affecting cell viability, proliferation, mitochondrial respiration or inflammatory status.
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影响因子：
3.7
作者：
Dykes J;Lenshof A;Åstrand-Grundström IB;Laurell T;Scheding S
通讯作者：
Scheding S
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