Decolorization and degradation of synthetic dyes by Schizophyllum sp. F17 in a novel system

Decolorization and degradation of synthetic dyes by Schizophyllum sp. F17 in a novel system
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裂褶藻对合成染料的脱色和降解。

DOI:
10.1016/j.desal.2010.07.024
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发表时间:
2011-01
期刊:
影响因子:
9.9
通讯作者:
荚荣
荚荣
中科院分区:
工程技术2区
文献类型:
--
作者:
荚荣

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染料废水的处理提出了一个主要问题和生物处理的白色腐真菌似乎是一个可行的选择。由于细菌和其他微生物的污染,真菌在非无菌条件下的废水处理中的应用受到限制。我们设计了一个新的系统,包括100 ml/L(0.01mol/L,pH4.0)乳酸缓冲液、100 mg/L染料和菌丝球(3.0g/L木屑),裂褶菌F17对不同结构的染料均有较好的脱色降解效果,对刚果、茜素红、中性红、结晶紫的脱色率分别达到92.4%、93.4%、120 h后,分别为83.6%和70.5%。在无菌和非无菌条件下考察了该体系对刚果红的脱色和降解,120 h后降解率分别达到83.2%和78.6%。刚果红在非无菌和无菌条件下的降解无明显差异。主要降解酶锰过氧化物酶的累积活性与脱色率呈良好的线性关系,相关系数为0.986。采用高效液相色谱法对刚果红的降解产物进行分离,鉴定为联苯胺。
Treatment of dye effluents poses a major problem and biotreatment by white rot fungus seems to be a viable option. The application of the fungus in wastewater treatment under non-sterile conditions has been limited owing to contamination by bacteria and other microorganisms. We designed a novel system comprising 100ml/L (0.01mol/L, pH 4.0) lactic acid buffer solution, 100mg/L dye and mycelial pellets (with 3.0g/L wood chips), in which Schizophyllum sp. F17 could effectively decolorize and degrade dyes having different structures, and the decolorization ratios of Congo Red, Alizarin Red, Neutral Red and Crystal Violet reached 92.4%, 93.4%, 83.6% and 70.5%, respectively, after 120h. The decolorization and degradation of Congo Red in the system were investigated under sterile and non-sterile conditions, and the degradation ratio reached 83.2% and 78.6% respectively, after 120h. No obvious difference was observed under non-sterile and sterile conditions in the degradation of Congo Red. There was a good linear relationship between the cumulative activity of manganese peroxidase (the main degradation enzyme) and the decolorization ratio, in which the correlation coefficient was 0.986. The degradation product of Congo Red was separated by high-performance liquid chromatography, and was identified as benzidine.
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