Specific immobilization of influenza A virus on GaAs (001) surface.
Specific immobilization of influenza A virus on GaAs (001) surface.
复制标题
甲型流感病毒在 GaAs (001) 表面的特异性固定。
DOI：
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发表时间：
2009
影响因子：
3.5
通讯作者：
J. Dubowski
中科院分区：
文献类型：
--
作者：
V. Duplan;Y. Miron;E. Frost;M. Grandbois;J. Dubowski
在寻求开发用于病毒病原体快速检测和分型的全光学生物传感器的过程中，我们研究了利用III-V量子半导体（QS）的强光致发光发射的生物传感架构。开发这种生物传感器的关键因素之一是能够将各种分析物附着到GaAs上-这是我们感兴趣的用于覆盖III-V QS的材料。我们报告了GaAs（001）与聚乙二醇（PEG）硫醇的生物功能化的研究和成功的固定化流感病毒A。使用生物素化PEG硫醇在OH-封端PEG硫醇中的稀释溶液形成用于连接中性抗生物素蛋白的位点网络。应用生物素标记的甲型流感多克隆抗体研究灭活甲型流感病毒的固定化过程。使用原子力显微镜和荧光显微镜测量证明了成功的固定化。
In the quest for the development of an all-optical biosensor for rapid detection and typing of viral pathogens, we investigate biosensing architectures that take advantage of strong photoluminescence emission from III-V quantum semiconductors (QS). One of the key elements in the development of such a biosensor is the ability to attach various analytes to GaAs--a material of choice for capping III-V QS of our interest. We report on the study of biofunctionalization of GaAs (001) with polyethylene-glycol (PEG) thiols and the successful immobilization of influenza A virus. A diluted solution of biotinylated PEG thiols in OH-terminated PEG thiols is used to form a network of sites for the attachment of neutravidin. Biotinylated polyclonal influenza A antibodies are applied to investigate the process of the immobilization of inactivated influenza A virus. The successful immobilization is demonstrated using atomic force microscopy and fluorescence microscopy measurements.
影响因子：
3.5
作者：
Gao, XH;Chan, WCW;Nie, SM
通讯作者：
Nie, SM