Quantitative analysis of single-molecule superresolution images.

Quantitative analysis of single-molecule superresolution images.
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DOI:
10.1016/j.sbi.2014.08.008
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发表时间:
2014-10
影响因子:
6.8
通讯作者:
Xiao, Jie
Xiao, Jie
中科院分区:
生物学2区
文献类型:
--
作者:
Coltharp, Carla;Yang, Xinxing;Xiao, Jie

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This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10 – 50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.
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