Spatial covariance reconstructive (SCORE) super-resolution fluorescence microscopy.
Spatial covariance reconstructive (SCORE) super-resolution fluorescence microscopy.
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DOI：
10.1371/journal.pone.0094807
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Shaevitz JW
中科院分区：
文献类型：
--
作者：
Deng Y;Sun M;Lin PH;Ma J;Shaevitz JW
超分辨率荧光显微镜已成为一个强大的工具，以解决传统的衍射限制成像技术，如共聚焦显微镜无法访问的结构信息。随机光学重建显微镜（STORM）和光活化定位显微镜（PALM）是有前途的超分辨率技术，由于其相对容易的实施和仪器的标准显微镜。然而，STORM的应用受到其长采样时间的严重限制。最近的几项工作都集中在提高STORM成像速度，通过利用来自具有重叠点扩散函数（PSF）的辐射源的信息。在这项工作中，我们提出了一个快速，高效的算法，考虑到独立的荧光发射器的闪烁统计。我们实现了100 nm的亚衍射横向分辨率从5到7秒的成像。我们的方法对背景不敏感，可以应用于不同类型的荧光源，包括但不限于我们在这项工作中展示的有机染料和量子点。
Super-resolution fluorescence microscopy has become a powerful tool to resolve structural information that is not accessible to traditional diffraction-limited imaging techniques such as confocal microscopy. Stochastic optical reconstruction microscopy (STORM) and photoactivation localization microscopy (PALM) are promising super-resolution techniques due to their relative ease of implementation and instrumentation on standard microscopes. However, the application of STORM is critically limited by its long sampling time. Several recent works have been focused on improving the STORM imaging speed by making use of the information from emitters with overlapping point spread functions (PSF). In this work, we present a fast and efficient algorithm that takes into account the blinking statistics of independent fluorescence emitters. We achieve sub-diffraction lateral resolution of 100 nm from 5 to 7 seconds of imaging. Our method is insensitive to background and can be applied to different types of fluorescence sources, including but not limited to the organic dyes and quantum dots that we demonstrate in this work.
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影响因子：
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作者：
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影响因子：
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