Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment
Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment
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由多个可调节氢键驱动的智能聚合物，可富集完整的磷蛋白
DOI：
10.1080/14686996.2019.1643259
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发表时间：
2019-07
影响因子：
5.5
通讯作者：
Xinmiao Liang
中科院分区：
文献类型：
--
作者：
Xiaofei Zhang;Qi Lu;Cheng Chen;Xiuling Li;Guangyan Qing;Taolei Sun;Xinmiao Liang
摘要 磷蛋白的分离对于理解它们在生物过程和病理学中的重要作用至关重要。基于过渡金属的受体和抗体是常用于磷蛋白富集的材料，但两者都存在……（原句此处不完整）
ABSTRACT Separation of phosphoproteins is essential for understanding their vital roles in biological processes and pathology. Transition metal-based receptors and antibodies, the routinely used materials for phosphoproteins enrichment, both suffer from low sensitivity, low recovery and coverage. In this work, a novel smart copolymer material was synthesized by modifying porous silica gel with a poly[(N-isopropylacrylamide-co-4-(3-acryloylthioureido) benzoic acid)0.35] (denoted as NIPAAm-co-ATBA0.35@SiO2). Driven by the hydrogen bonds complexation of ATBA monomers with phosphate groups, the copolymer-modified surface exhibited a remarkable adsorption toward native α-casein (a model phosphoprotein), accompanied with significant changes in surface viscoelasticity and roughness. Moreover, this adsorption was tunable and critically dependent on the polarity of carrier solvent. Benefiting from these features, selective enrichment of phosphoprotein was obtained using NIPAAm-co-ATBA0.35@SiO2 under a dispersive solid-phase extraction (dSPE) mode. This result displays a good potential of smart polymeric materials in phosphoprotein enrichment, which may facilitate top-down phosphoproteomics studies. GRAPHICAL ABSTRACT
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影响因子：
64.5
作者：
Antal CE;Hudson AM;Kang E;Zanca C;Wirth C;Stephenson NL;Trotter EW;Gallegos LL;Miller CJ;Furnari FB;Hunter T;Brognard J;Newton AC
通讯作者：
Newton AC
影响因子：
46.9
作者：
Rush, J;Moritz, A;Comb, MJ
通讯作者：
Comb, MJ
磁性聚苯乙炔共轭微孔聚合物微球用于超高效液相色谱-串联质谱富集和分析杀菌剂
影响因子：
4.1
作者：
Lei, Haoyu;Hu, Yuling;Li, Gongke
通讯作者：
Li, Gongke
影响因子：
6.7
作者：
Hu Y;Peng Y;Lin K;Shen H;Brousseau LC 3rd;Sakamoto J;Sun T;Ferrari M
通讯作者：
Ferrari M
影响因子：
4.2
作者：
Wu, Mingxue;Chen, Gang;Jia, Qiong
通讯作者：
Jia, Qiong