Genetic requirements and transcriptomics of Helicobacter pylori biofilm formation on abiotic and biotic surfaces.
Genetic requirements and transcriptomics of Helicobacter pylori biofilm formation on abiotic and biotic surfaces.
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幽门螺杆菌生物膜在非生物和生物表面形成的遗传要求和转录组学。
DOI：
10.1038/s41522-020-00167-3
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发表时间：
2020-11-27
影响因子：
9.2
通讯作者：
Ottemann KM
中科院分区：
文献类型：
--
作者：
Hathroubi S;Hu S;Ottemann KM
生物膜生长是一种广泛的机制，可以保护细菌免受恶劣环境，抗菌剂和免疫反应的影响。这些类型的条件挑战慢性定植者，如幽门螺杆菌，但它是不完全了解如何H。pylori生物膜生长的定义及其对H.幽门存活率。为深入了解H. pylori生物膜生长特性，我们表征了非生物和生物表面上的生物膜形成，鉴定了生物膜形成所需的基因，并定义了实验室模型H. pylori菌株G27。我们报告说，H。pylori G27在塑料和胃上皮细胞上形成具有高生物量和复杂的鞭毛填充的3D结构的生物膜。使用筛选生物膜缺陷突变体和转录组学，我们发现，生物膜细胞表现出较低的转录TCA循环酶，但较高的鞭毛形成，两种类型的四分泌系统，氢化酶和丙酮代谢。我们证实，生物膜的形成需要鞭毛，氢化酶和丙酮代谢的非生物和生物表面。总之，这些数据表明，H。幽门螺杆菌能够在作为生物膜生长时调整其表型，改变其代谢，并将鞭毛（通常为运动细胞器）重塑为粘附结构。
Biofilm growth is a widespread mechanism that protects bacteria against harsh environments, antimicrobials, and immune responses. These types of conditions challenge chronic colonizers such as Helicobacter pylori but it is not fully understood how H. pylori biofilm growth is defined and its impact on H. pylori survival. To provide insights into H. pylori biofilm growth properties, we characterized biofilm formation on abiotic and biotic surfaces, identified genes required for biofilm formation, and defined the biofilm-associated gene expression of the laboratory model H. pylori strain G27. We report that H. pylori G27 forms biofilms with a high biomass and complex flagella-filled 3D structures on both plastic and gastric epithelial cells. Using a screen for biofilm-defective mutants and transcriptomics, we discovered that biofilm cells demonstrated lower transcripts for TCA cycle enzymes but higher ones for flagellar formation, two type four secretion systems, hydrogenase, and acetone metabolism. We confirmed that biofilm formation requires flagella, hydrogenase, and acetone metabolism on both abiotic and biotic surfaces. Altogether, these data suggest that H. pylori is capable of adjusting its phenotype when grown as biofilm, changing its metabolism, and re-shaping flagella, typically locomotion organelles, into adhesive structures.
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影响因子：
6.4
作者：
Howitt MR;Lee JY;Lertsethtakarn P;Vogelmann R;Joubert LM;Ottemann KM;Amieva MR
通讯作者：
Amieva MR
影响因子：
4.8
作者：
Lécuyer F;Bourassa JS;Gélinas M;Charron-Lamoureux V;Burrus V;Beauregard PB
通讯作者：
Beauregard PB
影响因子：
5.2
作者：
De la Cruz, Miguel A.;Ares, Miguel A.;Torres, Javier
通讯作者：
Torres, Javier
影响因子：
12.6
作者：
Cammarota, Giovanni;Branca, Giovanna;Gasbarrini, Giovanni
通讯作者：
Gasbarrini, Giovanni
影响因子：
6.4
作者：
Gaddy, Jennifer A.;Radin, Jana N.;Algood, Holly M. S.
通讯作者：
Algood, Holly M. S.