Rab20 regulates phagosome maturation in RAW264 macrophages during Fc gamma receptor-mediated phagocytosis.
Rab20 regulates phagosome maturation in RAW264 macrophages during Fc gamma receptor-mediated phagocytosis.
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DOI：
10.1371/journal.pone.0035663
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Araki N
中科院分区：
文献类型：
--
作者：
Egami Y;Araki N
Rab20是rabgtpase家族的一员，已知参与细胞膜运输，但其在fc γ r介导的吞噬中的作用尚不清楚。我们检测了Rab20在RAW264巨噬细胞吞噬IgG-opsonized红细胞（IgG-Es）过程中的时空定位。通过荧光蛋白融合Rab20的活细胞成像，发现Rab20与吞噬体膜有短暂的结合。在吞噬体形成初期，Rab20并不局限于吞噬杯的膜上，而是逐渐被招募到新形成的吞噬体中。虽然Rab20在一定程度上与Rab5共定位，但即使Rab5从吞噬体膜上丢失，Rab20与吞噬体的关联仍然存在。然后，将Rab20与Rab7和Lamp1（内体/溶酶体晚期标记物）共定位在内化吞噬体上。此外，我们对Rab20突变体表达的分析显示，在表达gdp结合突变体Rab20- t19n的细胞中，吞噬体的成熟明显延迟。这些数据表明Rab20是吞噬小体的重要组成部分，并在fc γ r介导的吞噬过程中调控吞噬小体的成熟。
Rab20, a member of the Rab GTPase family, is known to be involved in membrane trafficking, however its implication in FcγR-mediated phagocytosis is unclear. We examined the spatiotemporal localization of Rab20 during phagocytosis of IgG-opsonized erythrocytes (IgG-Es) in RAW264 macrophages. By the live-cell imaging of fluorescent protein-fused Rab20, it was shown that Rab20 was transiently associated with the phagosomal membranes. During the early stage of phagosome formation, Rab20 was not localized on the membranes of phagocytic cups, but was gradually recruited to the newly formed phagosomes. Although Rab20 was colocalized with Rab5 to some extent, the association of Rab20 with the phagosomes persisted even after the loss of Rab5 from the phagosomal membranes. Then, Rab20 was colocalized with Rab7 and Lamp1, late endosomal/lysosomal markers, on the internalized phagosomes. Moreover, our analysis of Rab20 mutant expression revealed that the maturation of phagosomes was significantly delayed in cells expressing the GDP-bound mutant Rab20-T19N. These data suggest that Rab20 is an important component of phagosome and regulates the phagosome maturation during FcγR-mediated phagocytosis.
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影响因子：
7.8
作者：
Smith, Adam C.;Do Heo, Won;Brumell, John H.
通讯作者：
Brumell, John H.
影响因子：
4.8
作者：
Downey, GP;Botelho, RJ;Grinstein, S
通讯作者：
Grinstein, S
DOI：
10.1038/nrm2447
发表时间：
2008-08
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Egami Y;Araki N
通讯作者：
Araki N
影响因子：
3.7
作者：
Araki, Nobukazu;Egami, Youhei;Hatae, Tanenori
通讯作者：
Hatae, Tanenori