Eisosome proteins assemble into a membrane scaffold.
Eisosome proteins assemble into a membrane scaffold.
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DOI：
10.1083/jcb.201104040
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发表时间：
2011-11-28
期刊：
影响因子：
--
通讯作者：
Walther TC
中科院分区：
文献类型：
--
作者：
Karotki L;Huiskonen JT;Stefan CJ;Ziółkowska NE;Roth R;Surma MA;Krogan NJ;Emr SD;Heuser J;Grünewald K;Walther TC
由Eisosomes介导的膜组织是通过其主要成分自组装成具有脂质结合特异性的膜结合蛋白质支架。将膜空间组织成不同蛋白质和脂质组成的域是生物系统的基本特征。质膜被组织在这样的领域，以有效地协调同时发生的许多反应。尽管膜结构域几乎普遍存在，但其形成机制往往不清楚。酵母细胞具有突出的质膜结构域组织，这至少部分是由Eisosomes介导的。Eisosomes是大型蛋白质复合物，主要由两种含有Bin-Amphiphysin-Rvs结构域的蛋白质Pil 1和Lsp 1的许多亚基组成。在本文中，我们表明，这些蛋白质自组装成高阶结构，并优先结合到含磷酸肌醇的膜。使用电子显微镜的方法相结合，我们产生的Pil 1和Lsp 1组件的结构模型，这类似于细胞中的eisosomes。我们的数据表明，由eisosomes的膜组织的机制是介导的自组装成一个膜结合的蛋白质支架与脂质结合的特异性，其核心组件。
Membrane organization by eisosomes is mediated by self-assembly of its main components into a membrane-bound protein scaffold with lipid-binding specificity. Spatial organization of membranes into domains of distinct protein and lipid composition is a fundamental feature of biological systems. The plasma membrane is organized in such domains to efficiently orchestrate the many reactions occurring there simultaneously. Despite the almost universal presence of membrane domains, mechanisms of their formation are often unclear. Yeast cells feature prominent plasma membrane domain organization, which is at least partially mediated by eisosomes. Eisosomes are large protein complexes that are primarily composed of many subunits of two Bin–Amphiphysin–Rvs domain–containing proteins, Pil1 and Lsp1. In this paper, we show that these proteins self-assemble into higher-order structures and bind preferentially to phosphoinositide-containing membranes. Using a combination of electron microscopy approaches, we generate structural models of Pil1 and Lsp1 assemblies, which resemble eisosomes in cells. Our data suggest that the mechanism of membrane organization by eisosomes is mediated by self-assembly of its core components into a membrane-bound protein scaffold with lipid-binding specificity.
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DOI：
10.1083/jcb.200806035
发表时间：
2008-12-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Grossmann G;Malinsky J;Stahlschmidt W;Loibl M;Weig-Meckl I;Frommer WB;Opekarová M;Tanner W
通讯作者：
Tanner W
影响因子：
11.8
作者：
Chang-Ileto, Belle;Frere, Samuel G.;Chan, Robin B.;Voronov, Sergey V.;Roux, Aurelian;Di Paolo, Gilbert
通讯作者：
Di Paolo, Gilbert
迭代螺旋实空间重构法： 解决实际聚合物带来的问题
影响因子：
3
作者：
Egelman, Edward H.
通讯作者：
Egelman, Edward H.
影响因子：
5.6
作者：
Chacón, P;Wriggers, W
通讯作者：
Wriggers, W
DOI：
10.1042/bss0720223
发表时间：
2005-01-01
期刊：
LIPIDS, RAFTS AND TRAFFIC
影响因子：
--
作者：
Gallop, JL;McMahon, HT
通讯作者：
McMahon, HT