Phase Separation in Biology and Disease; Current Perspectives and Open Questions.
Phase Separation in Biology and Disease; Current Perspectives and Open Questions.
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DOI：
10.1016/j.jmb.2023.167971
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发表时间：
2023-03-01
影响因子：
5.6
通讯作者：
Kriwacki, Richard
中科院分区：
文献类型：
--
作者：
Boeynaems, Steven;Chong, Shasha;Gsponer, Jorg;Holt, Liam;Milovanovic, Dragomir;Mitrea, Diana M.;Mueller-Cajar, Oliver;Portz, Bede;Reilly, John F.;Reinkemeier, Christopher D.;Sabari, Benjamin R.;Sanulli, Serena;Shorter, James;Sontag, Emily;Strader, Lucia;Stachowiak, Jeanne;Weber, Stephanie C.;White, Michael;Zhang, Huaiying;Zweckstetter, Markus;Elbaum-Garfinkle, Shana;Kriwacki, Richard
在过去的近15年里，我们见证了一个新的科学领域的诞生，该领域专注于细胞中无膜体的存在、形成、生物功能和疾病关联，现在被称为生物分子凝聚体。来自几个实验室的开创性研究支持一个模型，在该模型中，与不同生物过程相关的生物分子凝聚物通过相分离过程形成。这些以及随后的其他发现彻底改变了我们对生物分子在细胞内如何在空间和时间上组织以执行无数生物学功能的理解，包括决定细胞命运、信号转导、内吞、基因表达和蛋白质翻译的调节以及RNA代谢的调节。此外，通过异常相变形成的冷凝物与许多人类疾病有关，特别是神经退化和癌症。虽然在某些情况下，严格的证据支持通过相分离形成生物分子凝聚体与生物功能之间的联系，但在许多其他情况下，这种联系没有得到那么有力的支持，这导致了对相分离在生物学和疾病中作用的一般性的正当审查。2022年3月，在科罗拉多州泰勒里德的Telluride科学研究中心(TSRC)举行的为期一周的研讨会上，约25名科学家讨论了围绕生物分子凝析油领域的关键问题。在这里，我们介绍了通过这些讨论获得的见解，涉及的主题包括，缩合物在不同生物过程和系统中的作用，正常和疾病细胞状态，它们在合成生物学中的应用，以及治疗靶向生物分子缩合物的可能性。
In the past almost 15 years, we witnessed the birth of a new scientific field focused on the existence, formation, biological functions, and disease associations of membraneless bodies in cells, now referred to as biomolecular condensates. Pioneering studies from several laboratories [reviewed in ] supported a model wherein biomolecular condensates associated with diverse biological processes form through the process of phase separation. These and other findings that followed have revolutionized our understanding of how biomolecules are organized in space and time within cells to perform myriad biological functions, including cell fate determination, signal transduction, endocytosis, regulation of gene expression and protein translation, and regulation of RNA metabolism. Further, condensates formed through aberrant phase transitions have been associated with numerous human diseases, prominently including neurodegeneration and cancer. While in some cases, rigorous evidence supports links between formation of biomolecular condensates through phase separation and biological functions, in many others such links are less robustly supported, which has led to rightful scrutiny of the generality of the roles of phase separation in biology and disease. During a week-long workshop in March 2022 at the Telluride Science Research Center (TSRC) in Telluride, Colorado, ~25 scientists addressed key questions surrounding the biomolecular condensates field. Herein, we present insights gained through these discussions, addressing topics including, roles of condensates in diverse biological processes and systems, and normal and disease cell states, their applications to synthetic biology, and the potential for therapeutically targeting biomolecular condensates.
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影响因子：
3.6
作者：
Cabrera, JE;Jin, DJ
通讯作者：
Jin, DJ
YAP的相位分离重新组织了基因组拓扑，以进行长期YAP靶基因表达。
影响因子：
21.3
作者：
Cai D;Feliciano D;Dong P;Flores E;Gruebele M;Porat-Shliom N;Sukenik S;Liu Z;Lippincott-Schwartz J
通讯作者：
Lippincott-Schwartz J
相位分离驱动异常的染色质循环和癌症发展。
影响因子：
64.8
作者：
Ahn JH;Davis ES;Daugird TA;Zhao S;Quiroga IY;Uryu H;Li J;Storey AJ;Tsai YH;Keeley DP;Mackintosh SG;Edmondson RD;Byrum SD;Cai L;Tackett AJ;Zheng D;Legant WR;Phanstiel DH;Wang GG
通讯作者：
Wang GG
影响因子：
7.7
作者：
Cho, Won-Ki;Jayanth, Namrata;Cisse, Ibrahim I.
通讯作者：
Cisse, Ibrahim I.
影响因子：
5.6
作者：
Dai, Wei;Chen, Muyuan;Chiu, Wah
通讯作者：
Chiu, Wah