Elucidating drug targets and mechanisms of action by genetic screens in mammalian cells.
Elucidating drug targets and mechanisms of action by genetic screens in mammalian cells.
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DOI：
10.1039/c7cc02349a
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发表时间：
2017-06-29
期刊：
影响因子：
--
通讯作者：
Kampmann M
中科院分区：
文献类型：
--
作者：
Kampmann M
Phenotypic screening is a powerful approach to discover small molecules with desired effects on biological systems, which can then be developed into therapeutic drugs. The identification of the target and mechanism of action of compounds discovered in phenotypic screens remains a major challenge. This Feature Article describes the use of genetic tools to reveal drug targets and mechanisms in mammalian cells. Until recently, RNA interference was the method of choice for such studies. Here, we highlight very recent additions to the genetic toolkit in mammalian cells, including CRISPR, CRISPR interference, and CRISPR activation, and we illustrate their usefulness for drug target identification.
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影响因子：
7.7
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Acosta-Alvear D;Cho MY;Wild T;Buchholz TJ;Lerner AG;Simakova O;Hahn J;Korde N;Landgren O;Maric I;Choudhary C;Walter P;Weissman JS;Kampmann M
通讯作者：
Kampmann M
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Charpentier, Emmanuelle
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Schenone M;Dančík V;Wagner BK;Clemons PA
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Clemons PA
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14.8
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Julien, Olivier;Kampmann, Martin;Bassik, Michael C.;Zorn, Julie A.;Venditto, Vincent J.;Shimbo', Kazutaka;Agard, Nicholas J.;Shimada, Kenichi;Rheingold, Arnold L.;Stockwell, Brent R.;Weissman, Jonathan S.;Wells, James A.
通讯作者：
Wells, James A.
DOI：
10.1073/pnas.1508821112
发表时间：
2015-06-30
影响因子：
11.1
作者：
Kampmann, Martin;Horlbeck, Max A.;Weissman, Jonathan S.
通讯作者：
Weissman, Jonathan S.