Functional characterization of PhGR and PhGRL1 during flower senescence in the petunia
Functional characterization of PhGR and PhGRL1 during flower senescence in the petunia
复制标题
矮牵牛花衰老过程中 PhGR 和 PhGRL1 的功能特征
DOI：
10.1007/s00299-015-1808-7
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发表时间：
2015-05
影响因子：
6.2
通讯作者：
Yu Yixun
中科院分区：
文献类型：
--
作者：
Tan Yinyan;Zhong Shan;Chen Guoju;Yu Yixun
PhGRL1抑制加速了矮牵牛花的衰老，增加了乙烯信号下游基因的表达，而PhGR抑制则没有。乙烯在花的衰老过程中起着重要的作用。同源蛋白绿熟（Green-Ripe）和乙烯敏感性还原（Reversion to Ethylene sensitivity）分别是番茄和拟南芥乙烯响应的正调节因子。矮牵牛花已被用作研究衰老过程中乙烯反应的模型。本研究分析了牵牛aphgrandphgrl1在不同器官、花衰老过程和外源乙烯处理后的表达；研究了PhGR和PhGRL1在矮牵牛花衰老过程中的作用。病毒诱导的基因沉默介导的PhGRL1抑制加速了花的衰老和乙烯产量的增加；而对PhGR没有抑制作用。综上所述，这些数据表明PhGRL1参与了花衰老的负调控，可能是通过抑制乙烯产生，从而降低乙烯信号的激活。
Key messagePetunia PhGRL1 suppression accelerated flower senescence and increased the expression of the genes downstream of ethylene signaling, whereas PhGR suppression did not.AbstractEthylene plays an important role in flowers senescence. Homologous proteins Green-Ripe and Reversion to Ethylene sensitivity1 are positive regulators of ethylene responses in tomato andArabidopsis, respectively. The petunia flower has served as a model for the study of ethylene response during senescence. In this study, petuniaPhGRandPhGRL1expression was analyzed in different organs, throughout floral senescence, and after exogenous ethylene treatment; and the roles of PhGR and PhGRL1 during petunia flower senescence were investigated. PhGRL1 suppression mediated by virus-induced gene silencing accelerated flower senescence and increased ethylene production; however, the suppression of PhGR did not. Taken together, these data suggest that PhGRL1 is involved in negative regulation of flower senescence, possibly via ethylene production inhibition and consequently reduced ethylene signaling activation.
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影响因子：
27.5
作者：
S. Luan;Xiao-Ya Chen;N. Raikhel;W. Briggs
通讯作者：
S. Luan;Xiao-Ya Chen;N. Raikhel;W. Briggs
影响因子：
7.4
作者：
Underwood, BA;Tieman, DM;Clark, DG
通讯作者：
Clark, DG
DOI：
10.1126/science.1225974
发表时间：
2012-10-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Qiao H;Shen Z;Huang SS;Schmitz RJ;Urich MA;Briggs SP;Ecker JR
通讯作者：
Ecker JR
DOI：
10.1073/pnas.0602319103
发表时间：
2006-05-16
影响因子：
11.1
作者：
Barry, Cornelius S.;Giovannoni, James J.
通讯作者：
Giovannoni, James J.
影响因子：
64.5
作者：
Potuschak, T;Lechner, E;Genschik, P
通讯作者：
Genschik, P