senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence.
senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence.
复制标题
DOI：
10.1186/s12866-014-0265-8
复制
发表时间：
2014-10-25
期刊：
影响因子：
4.2
通讯作者：
Karakousis PC
中科院分区：
文献类型：
--
作者：
Rifat D;Belchis DA;Karakousis PC
Mycobacterium tuberculosis (Mtb) must adapt to various stress conditions during host infection. The two-component regulatory system (2CRS) SenX3-RegX3 is required for Mtb virulence. We showed recently that the senX3-regX3 intergenic region contains promoter activity, driving senX3-independent regX3 expression. In the current study, we tested the hypothesis that RegX3 has a SenX3-independent role in Mtb virulence. The gene expression patterns, growth, and survival of mutants containing transposon insertions in senX3 (senX3::Tn) and regX3 (regX3::Tn) were compared to those of their respective complemented strains and the isogenic wild-type parent strain during axenic growth in nutrient-rich broth, phosphate depletion, nutrient starvation, and in the lungs of BALB/c mice. regX3 expression was reduced in senX3::Tn during phosphate depletion and nutrient starvation, and expression of the phosphate-specific transport gene pstC2 was reduced similarly in senX3::Tn and regX3::Tn during phosphate depletion. Although senX3 and regX3 were each dispensable for Mtb growth in nutrient-rich broth, disruption of senX3 or regX3 caused a similar growth defect during phosphate depletion. Interestingly, senX3::Tn, in which monocistronic regX3 expression is preserved, showed significantly higher survival relative to regX3::Tn after 7 days of nutrient starvation (p <0.01), and in mouse lungs at Day 31 (p < 0.01), Day 62 (p < 0.01), and Day 124 (p = 0.05) after aerosol infection. Our data demonstrate the specificity of the senX3-regX3 2CRS for sensing and responding to low ambient phosphate, but also raise the possibility that RegX3 may function independently of its cognate sensor histidine kinase.
登录
查看更多内容
DOI：
10.1086/656524
发表时间：
2010-11-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Klinkenberg LG;Lee JH;Bishai WR;Karakousis PC
通讯作者：
Karakousis PC
影响因子：
64.5
作者：
BURBULYS, D;TRACH, KA;HOCH, JA
通讯作者：
HOCH, JA
影响因子：
3.2
作者：
Batchelor, E;Walthers, D;Goulian, M
通讯作者：
Goulian, M
DOI：
10.1073/pnas.1631248100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Dahl, JL;Kraus, CN;Barry, CE
通讯作者：
Barry, CE
影响因子：
3.2
作者：
BALDUS, JM;GREEN, BD;MORAN, CP
通讯作者：
MORAN, CP