Identification and characterization of a Ste20-like kinase in Artemia and its role in the developmental regulation and resistance to environmental stress.
Identification and characterization of a Ste20-like kinase in Artemia and its role in the developmental regulation and resistance to environmental stress.
复制标题
卤虫中 Ste20 样激酶的鉴定和表征及其在发育调节和环境应激抵抗中的作用
DOI：
10.1371/journal.pone.0092234
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yang F
中科院分区：
文献类型：
--
作者：
Zhou R;Sun YX;Yang WJ;Yang F
为了适应极端的环境，甲壳类动物阿耳特米亚进化出了两种可选择的生殖途径。在卵胎生（直接）发育过程中，产生无尾螺幼虫。相比之下，雌性蒿则通过卵生途径释放被包裹的滞育胚胎（囊肿）。迄今为止，调控青蒿抗逆性的细胞机制在很大程度上仍然未知。ste20样激酶（SLK）参与多种生物过程，包括应激反应、细胞凋亡和细胞周期进程。我们从单性生殖蒿中分离到一个名为ArSLK的SLK超家族成员并进行了鉴定。ArSLK基因在卵胎生和卵生发育过程中都有转录；然而，该蛋白主要位于抗逆性滞育囊的细胞核中，而不像无裂胚和无裂胚的细胞质中。有趣的是，暴露于热休克、酸性pH和紫外线照射下的nauplii诱导ArSLK从细胞质转移到细胞核。这种易位在去除压力后被逆转。此外，在生理应激条件下，内源性ArSLK基因被RNAi敲低后，成虫产生的鹦鹉螺幼虫比注入GFP dsRNA的对照幼虫更早丧失自由游动能力。综上所述，本研究表明ArSLK在细胞质和细胞核之间的转运参与了青蒿的抗逆性调控。我们的发现可能对SLK超家族成员的功能提供重要的见解。
To adapt to extreme environments, the crustacean Artemia has evolved two alternative reproductive pathways. During ovoviviparous (direct) development, nauplius larvae are produced. In contrast, Artemia females release encysted diapause embryos (cysts) via the oviparous pathway. To date, the cellular mechanisms that regulate stress resistance of Artemia remain largely unknown. Ste20-like kinase (SLK) participates in multiple biological processes, including stress responses, apoptosis, and cell cycle progression. We isolated and characterized a member of the SLK superfamily termed ArSLK from Artemia parthenogenetica. The ArSLK gene is transcribed throughout both ovoviviparous and oviparous development; however, the protein is located mainly in the nuclei of stress-resistant diapause cysts, unlike the nauplii and nauplius-destined embryos where it is cytoplasmic. Interestingly, exposure of nauplii to heat shock, acidic pH, and UV irradiation induced the translocation of ArSLK from cytoplasm to nucleus. This translocation was reversed following stress removal. Moreover, under physiologically-stressful conditions, the nauplius larvae produced by adults after gene knockdown of endogenous ArSLK by RNAi, lost the ability of free-swimming much earlier than those of control larvae from females injected with GFP dsRNA. Taken together, this study demonstrated that trafficking of ArSLK between the cytoplasm and the nucleus participates in regulating the stress resistance of Artemia. Our findings may provide significant insight into the functions of members of the SLK superfamily.
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DOI：
10.1046/j.1432-1033.2003.03373.x
发表时间：
2003-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Chen, T;Amons, R;MacRae, TH
通讯作者：
MacRae, TH
影响因子：
3.7
作者：
Dai L;Chen DF;Liu YL;Zhao Y;Yang F;Yang JS;Yang WJ
通讯作者：
Yang WJ
影响因子：
3.7
作者：
Alves, PS;Godinho, SA;Tavares, AA
通讯作者：
Tavares, AA
影响因子：
4.8
作者：
Dai, Jie-Qiong;Zhu, Xiao-Jing;Yang, Wei-Jun
通讯作者：
Yang, Wei-Jun
影响因子：
2.4
作者：
Herbert, NA;Steffensen, JF
通讯作者：
Steffensen, JF