KISS1 gene expression in the developing brain of female pigs in pre- and peripubertal periods.
KISS1 gene expression in the developing brain of female pigs in pre- and peripubertal periods.
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DOI：
10.1262/jrd.2013-129
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Tsukamura H
中科院分区：
文献类型：
--
作者：
Ieda N;Uenoyama Y;Tajima Y;Nakata T;Kano M;Naniwa Y;Watanabe Y;Minabe S;Tomikawa J;Inoue N;Matsuda F;Ohkura S;Maeda K;Tsukamura H
青春期与促性腺激素释放激素(GnRH)分泌增加导致促性腺激素分泌增加有关。Kispeptin被认为在许多哺乳动物的青春期启动中起关键作用，包括啮齿动物、反刍动物和灵长类动物。本研究旨在确定编码Kispeptin的KISS1基因在下丘脑的表达变化是否与猪青春期的开始有关。每组动物4只，分别于0、1、2、3、4月龄，作为青春期前，5月龄作为围青春期，每次采血后用4%多聚甲醛灌流。用原位杂交技术观察Kiss1基因在脑冠状切片中的表达。放射免疫法测定血浆黄体生成素(L H)水平。在各个年龄段的弓状核(ARC)中都观察到了Kiss1mRNA信号，但表达KISS1的细胞数量没有显著差异，这表明KISS1基因在猪青春期发育过程中一直在ARC中表达。0月龄仔猪血浆促黄体生成素水平最高，1月龄和2月龄显著下降(P<；0.05)，提示青春期前促性腺激素释放存在负反馈机制。考虑到Kispeptin对青春期前猪促性腺激素释放的强烈刺激作用，Kispeptin的分泌而不是Kispeptin的合成可能是导致猪青春期开始的原因。
Puberty is associated with an increase in gonadotropin secretion as a result of an increase in gonadotropin-releasing hormone (GnRH) secretion. Kisspeptin is considered to play a key role in puberty onset in many mammalian species, including rodents, ruminants and primates. The present study aimed to determine if changes in hypothalamic expression of the KISS1 gene, encoding kisspeptin, are associated with the onset of puberty in pigs. The animals (n=4 in each group) were perfused with 4% paraformaldehyde at 0, 1, 2, 3 and 4 months old, as prepubertal stages, and at 5 months old, as the peripubertal stage, following each blood sampling. KISS1 gene expressions in coronal sections of brains were visualized by in situ hybridization. Plasma luteinizing hormone (LH) was measured by radioimmunoassay. KISS1 mRNA signals were observed in the arcuate nucleus (ARC) at all ages examined without any significant difference in the number of KISS1-expressing cells, indicating that the KISS1 gene is constantly expressed in the ARC throughout pubertal development in pigs. The plasma LH concentration was the highest in 0-month-old piglets and significantly decreased in the 1- and 2 month-old groups (P<0.05), suggesting a developing negative feedback mechanism affecting gonadotropin release during the prepubertal period. Considering the potent stimulating effect of kisspeptin on gonadotropin release in prepubertal pigs, kisspeptin secretion rather than kisspeptin synthesis may be responsible for the onset of puberty in pigs.
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DOI：
10.1073/pnas.1834399100
发表时间：
2003-09-16
影响因子：
11.1
作者：
de Roux, N;Genin, E;Milgrom, E
通讯作者：
Milgrom, E
影响因子：
4.8
作者：
de Tassigny, Xavier d'Anglemont;Fagg, Lisa A.;Colledge, William H.
通讯作者：
Colledge, William H.
DOI：
10.1073/pnas.0409822102
发表时间：
2005-02-08
影响因子：
11.1
作者：
Shahab, M;Mastronardi, C;Plant, TM
通讯作者：
Plant, TM
影响因子：
4.8
作者：
WATANABE, G;TERASAWA, E
通讯作者：
TERASAWA, E
影响因子：
1.8
作者：
Adachi, Sachika;Yamada, Shunji;Maeda, Kei-Ichiro
通讯作者：
Maeda, Kei-Ichiro