Issue Highlights November 2012
Issue Highlights November 2012
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2012 年 11 月发行要点
DOI：
10.1002/cyto.b.21051
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
F. Preffer
中科院分区：
文献类型：
--
作者：
F. Preffer
用细胞化学染色处理的血涂片检查几十年来一直被用作基本的诊断工具。然而，人工分类计数是时间和劳动密集型的，由于所评估的细胞数量相对较少，提供了对罕见群体的特别差的计数，遭受载玻片上细胞的不均匀分布，并且可能对具有不寻常形态的细胞进行错误分类。自动血细胞计数器在上个世纪中期成为实用工具，目前，全血细胞计数与白色细胞分类的组合可能是最需要的临床测试之一。本杂志以前的一篇报道中曾尝试用基于免疫表型的流动技术来改进这种分析（1）。在这个问题上，论文由Reynsel等。（2）是有关前瞻性的新方法，以获得更多的信息，从白色血细胞的区别。也就是说，这些作者试图从基于流式细胞术的白色细胞分类中进一步提取信息，以识别感染、脓毒症和/或脓毒性休克病例。而不是目前使用的7个参数中的白细胞分类，50个参数提供的报告仪器进行了分析，分层聚类的多维方式从459例重症监护病房。他们的结果在诊断感染、脓毒症和脓毒性休克病例的能力方面是有趣的，并强调了对可能提供额外重要标志物的扩展WBC分类的考虑。树突状细胞在脓毒症中的贡献和相关作用在本研究中进行了讨论，并在其他已发表的论文中进行了研究（3）。另外，扩展和应用这些方法来检查脑脊液（4）并进一步确定如何将基于流式细胞术的WBC的使用更好地整合到常规血液学实验室工作流程中也是非常有趣的。
A NEW LOOK AT THE LEUKOCYTE DIFFERENTIAL The examination of blood smears processed with cytochemical stains has been used as a fundamental diagnostic tool for decades. However, manual differential counts are time and labor intensive, provide particularly poor enumeration of infrequent populations due to the relatively small number of cells evaluated, suffer from nonuniform distribution of cells on the slide, and can misclassify cells having unusual morphology. Automated blood cell counters became practical tools midway through the last century, and currently, the combination of the complete blood cell count with white cell differential is likely among the most requested clinical test. An attempt at improving this analysis with an immunophenotypic flow-based technique has been the subject of a previous report in this journal (1). In this issue, the paper by Roussel et al. (2) is related to prospective new ways to acquire more information from the white blood cell differential. That is, it is an attempt by these authors to further extract information from a flow cytometricbased white cell differential that would identify cases of infection, sepsis and/or septic shock. Rather than the seven parameters currently used in a WBC differential, 50 parameters provided by the reported instrumentation were analyzed by hierarchical clustering in a multidimensional manner from 459 patients admitted to an intensive care unit. Their results are intriguing in their capacity to diagnose cases of infection, sepsis, and septic shock, and underscore the consideration of an extended WBC differential that may provide additional important markers. The contributing and relevant role of dendritic cells in sepsis is discussed in this study and has also been examined in other published papers (3). It would be additionally intriguing to extend and apply these methods to examine cerebral spinal fluid (4) and ascertain further how the use of a flow cytometric-based WBC be more integrated into routine hematology laboratory workflow.
影响因子：
1.7
作者：
Basiji, David A.;Ortyn, William E.;Morrissey, Philip
通讯作者：
Morrissey, Philip