Enhanced expression of the beta II subspecies of protein kinase C in differentiating erythroleukemia cells.
Enhanced expression of the beta II subspecies of protein kinase C in differentiating erythroleukemia cells.
复制标题
蛋白激酶 C β II 亚种在分化红白血病细胞中的表达增强。
DOI：
10.1016/0014-4827(90)90021-2
复制
发表时间：
1990
影响因子：
3.7
通讯作者：
Fermin,C
中科院分区：
文献类型：
--
作者：
Beckman,BS;Mason-Garcia,M;Martinez,MI;Fermin,C
Polyclonal antipeptide antibodies which recognize selected isozymes (α, β I, β II, and γ) of the protein kinase C family were used to identify specific subspecies in undifferentiated Friend erythroleukemia cells and in cells triggered to differentiate with hexamethylene bisacetamide. The β II isozyme of protein kinase C was the primary isozyme expressed and its abundance was significantly increased (P< 0.05) in differentiated cells. Differences in immunostaining between control and experimental groups were objectively quantitated by determining percentage transmission of light through cells based on color threshold rather than gray intensity levels. Staining was localized to the cytoplasm predominantly in differentiated cells, whereas nuclei stained more intensely in undifferentiated cells. These results provide immunocytochemical evidence to support the hypothesis that changes in the expression of the β II subspecies of protein kinase C are essential to the programmed maturation of differentiating Friend erythroleukemia cells.
登录
查看更多内容
DOI：
10.1111/j.1432-1033.1985.tb08854.x
发表时间：
1985-05
期刊：
European journal of biochemistry
影响因子：
--
作者：
G. Skoglund;A. Hansson;M. Ingelman-Sundberg
通讯作者：
G. Skoglund;A. Hansson;M. Ingelman-Sundberg
蛋白激酶C亚种在人白血病淋巴瘤细胞系中的表达
影响因子：
3.5
作者：
S. Sawamura;Katsuhiko Ase;N. Berry;U. Kikkawa;P. Mccaffrey;J. Minowada;Y. Nishizuka
通讯作者：
Y. Nishizuka
佛波酯介导的蛋白激酶 C 与 NIH 3T3 细胞核部分的关联。
影响因子：
11.2
作者：
T. P. Thomas;H. Talwar;W. Anderson
通讯作者：
W. Anderson
六亚甲基双乙酰胺诱导转化细胞分化：分子和细胞效应及治疗应用。
DOI：
10.1002/stem.5530060402
发表时间：
1988
期刊：
International journal of cell cloning
影响因子：
--
作者：
Marks,PA;Rifkind,RA
通讯作者：
Rifkind,RA
蛋白激酶C活性的变化与Friend红白血病细胞的分化相关。
DOI：
10.1016/0167-4889(87)90141-8
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Balazovich,KJ;Portnow,D;Boxer,LA;Prochownik,EV
通讯作者：
Prochownik,EV