Ecological genomics of Boechera stricta: identification of a QTL controlling the allocation of methionine- vs branched-chain amino acid-derived glucosinolates and levels of insect herbivory.
Ecological genomics of Boechera stricta: identification of a QTL controlling the allocation of methionine- vs branched-chain amino acid-derived glucosinolates and levels of insect herbivory.
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DOI：
10.1038/hdy.2009.12
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发表时间：
2009-05
期刊：
影响因子：
3.8
通讯作者：
Mitchell-Olds, T.
中科院分区：
文献类型：
--
作者：
Schranz, M. E.;Manzaneda, A. J.;Windsor, A. J.;Clauss, M. J.;Mitchell-Olds, T.
在十字花科中，硫代葡萄糖苷影响许多昆虫草食动物的取食、繁殖和发育。在模式植物拟南芥和油菜中，硫代葡萄糖苷的产生及其对食草动物摄食的影响已经得到了广泛的研究，这两种作物都能产生主要来自氨基酸蛋氨酸的叶片硫代葡萄糖苷。对其他脂肪族氨基酸如支链氨基酸(BCAA)、缬氨酸和异亮氨酸衍生的硫代葡萄糖苷的调节或防御作用知之甚少。我们已经鉴定出一种硫代葡萄糖苷多态，它控制着叶和种子中支链氨基酸和蛋氨酸衍生的硫代葡萄糖苷的分配。狭叶卷柏是一种多年生物种，生长在北美西部大部分未受干扰的栖息地。我们已经测量了192个F2品系的硫代葡萄糖苷图谱和浓度，这些品系以前曾用于构建遗传图谱。我们还对每个F2系的6个F3重复进行了食草性分析，使用的是多面手鳞翅目毛翅目。数量性状基因座(QTL)分析确定了一个控制硫代葡萄糖苷谱和草食水平的单一基因座，即支链蛋氨酸分配或BCMA QTL。我们已经将这个QTL限定在一个1.0LOD可信区间仅为1.9 cM的小基因组区域，在拟南芥中包含∼100基因。我们还发现，甲硫氨酸衍生的硫代葡萄糖苷比支链氨基酸衍生的硫代葡萄糖苷对这种多面性食草性昆虫的取食提供了显著更强的防御。未来对该基因座的位置克隆将允许测试各种适应性解释。
In the Brassicaceae glucosinolates influence feeding, reproduction and development of many insect herbivores. Glucosinolate production and effects on herbivore feeding have been extensively studied in the model species Arabidopsis thaliana and Brassica crops, both of which constitutively produce leaf glucosinolates mostly derived from the amino acid methionine. Much less is known about the regulation or role in defense of glucosinolates derived from other aliphatic amino acids such as the branched-chain amino acids (BCAA) valine and isoleucine. We have identified a glucosinolate polymorphism in Boechera stricta controlling the allocation to BCAA- vs. methionine-derived glucosinolates in both leaves and seeds. Boechera stricta is a perennial species that grows in mostly undisturbed habitats of western North America. We have measured glucosinolate profiles and concentrations in 192 F2 lines that have previously been used for genetic map construction. We also performed herbivory assays on six F3 replicates per F2 line using the generalist lepidopteran Trichoplusia ni. Quantitative Trait Locus (QTL) analysis identified a single locus controlling both glucosinolate profile and levels of herbivory, the Branched Chain-Methionine Allocation or BCMA QTL. We have delimited this QTL to a small genomic region with a 1.0 LOD confidence interval just 1.9 cM wide, which in A. thaliana contains ∼100 genes. We also found that methionine-derived glucosinolates provided significantly greater defense than the BCAA-derived glucosinolates against feeding by this generalist insect herbivore. The future positional cloning of this locus will allow for testing various adaptive explanations.
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影响因子：
7.4
作者：
de Kraker, Jan-Willem;Luck, Katrin;Gershenzon, Jonathan
通讯作者：
Gershenzon, Jonathan
影响因子：
7.2
作者：
Chen, SX;Glawischnig, E;Halkier, BA
通讯作者：
Halkier, BA
影响因子：
7.2
作者：
Gigolashvili, Tamara;Berger, Bettina;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
11.6
作者：
Hemm, MR;Ruegger, MO;Chapple, C
通讯作者：
Chapple, C
影响因子：
7.2
作者：
Hansen, Bjarne G.;Kliebenstein, Daniel J.;Halkier, Barbara A.
通讯作者：
Halkier, Barbara A.