Molecular surveillance for polymorphisms associated with artemisinin-based combination therapy resistance in Plasmodium falciparum isolates collected in Mozambique, 2018.
Molecular surveillance for polymorphisms associated with artemisinin-based combination therapy resistance in Plasmodium falciparum isolates collected in Mozambique, 2018.
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DOI：
10.1186/s12936-021-03930-9
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发表时间：
2021-10-12
期刊：
影响因子：
3
通讯作者：
Macete E
中科院分区：
文献类型：
--
作者：
Chidimatembue A;Svigel SS;Mayor A;Aíde P;Nhama A;Nhamussua L;Nhacolo A;Bassat Q;Salvador C;Enosse S;Saifodine A;De Carvalho E;Candrinho B;Zulliger R;Goldman I;Udhayakumar V;Lucchi NW;Halsey ES;Macete E
由于出现抗疟疾耐药性的威胁，世界卫生组织建议将抗疟疾耐药性分子标记监测纳入常规治疗疗效研究（TESs）。2018年，在莫桑比克开展了蒿甲醚-甲苯胺（AL）和青蒿琥酯-阿莫地喹（ASAQ）的TES检测，并调查了pfk13、pfcrt和pfmdr1耐药相关基因多态性的流行情况。年龄6-59个月的儿童被纳入四个研究地点。收集了在最初疟疾治疗28天内出现发热的参与者的血液，并用滤纸干燥。所有样本首先使用多重实时PCR法筛选恶性疟原虫，并通过Sanger测序研究pfk13、pfcrt和pfmdr1基因的多态性。未观察到与青蒿素部分耐药相关的pfk13突变。唯一观察到的pfcrt单倍型是野生型CVMNK（密码子72-76），与氯喹敏感性相关。pfmdr1仅在密码子184处存在多态性，突变型184F出现在43/109处（39.4%），野生型Y184出现在42/109处（38.5%），混合型184F/Y出现在24/109处（22.0%）。所有样品在这两个密码子上均含有N86和D1246。2018年，没有记录到青蒿素耐药性的标志物。分子监测应继续监测这些标志物的流行情况，以便为莫桑比克的疟疾治疗决策提供信息。
Due to the threat of emerging anti-malarial resistance, the World Health Organization recommends incorporating surveillance for molecular markers of anti-malarial resistance into routine therapeutic efficacy studies (TESs). In 2018, a TES of artemether-lumefantrine (AL) and artesunate-amodiaquine (ASAQ) was conducted in Mozambique, and the prevalence of polymorphisms in the pfk13, pfcrt, and pfmdr1 genes associated with drug resistance was investigated. Children aged 6–59 months were enrolled in four study sites. Blood was collected and dried on filter paper from participants who developed fever within 28 days of initial malaria treatment. All samples were first screened for Plasmodium falciparum using a multiplex real-time PCR assay, and polymorphisms in the pfk13, pfcrt, and pfmdr1 genes were investigated by Sanger sequencing. No pfk13 mutations, associated with artemisinin partial resistance, were observed. The only pfcrt haplotype observed was the wild type CVMNK (codons 72–76), associated with chloroquine sensitivity. Polymorphisms in pfmdr1 were only observed at codon 184, with the mutant 184F in 43/109 (39.4%) of the samples, wild type Y184 in 42/109 (38.5%), and mixed 184F/Y in 24/109 (22.0%). All samples possessed N86 and D1246 at these two codons. In 2018, no markers of artemisinin resistance were documented. Molecular surveillance should continue to monitor the prevalence of these markers to inform decisions on malaria treatment in Mozambique.
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影响因子：
11.8
作者：
Bergmann C;van Loon W;Habarugira F;Tacoli C;Jäger JC;Savelsberg D;Nshimiyimana F;Rwamugema E;Mbarushimana D;Ndoli J;Sendegeya A;Bayingana C;Mockenhaupt FP
通讯作者：
Mockenhaupt FP
影响因子：
11.8
作者：
Gupta H;Macete E;Bulo H;Salvador C;Warsame M;Carvalho E;Ménard D;Ringwald P;Bassat Q;Enosse S;Mayor A
通讯作者：
Mayor A
影响因子：
3.7
作者：
Gupta H;Galatas B;Chidimatembue A;Huijben S;Cisteró P;Matambisso G;Nhamussua L;Simone W;Bassat Q;Ménard D;Ringwald P;Rabinovich NR;Alonso PL;Saúte F;Aide P;Mayor A
通讯作者：
Mayor A
影响因子：
11.8
作者：
Halsey ES;Venkatesan M;Plucinski MM;Talundzic E;Lucchi NW;Zhou Z;Mandara CI;Moonga H;Hamainza B;Beavogui AH;Kariuki S;Samuels AM;Steinhardt LC;Mathanga DP;Gutman J;Denon YE;Uwimana A;Assefa A;Hwang J;Shi YP;Dimbu PR;Koita O;Ishengoma DS;Ndiaye D;Udhayakumar V
通讯作者：
Udhayakumar V
影响因子：
3
作者：
Ishengoma, Deus S.;Mandara, Celine I.;Udhayakumar, Venkatachalam
通讯作者：
Udhayakumar, Venkatachalam