Microorganisms associated with Sporobolus anglicus, an invasive dimethylsulfoniopropionate producing salt marsh plant, are an unrecognized sink for dimethylsulfide.
Microorganisms associated with Sporobolus anglicus, an invasive dimethylsulfoniopropionate producing salt marsh plant, are an unrecognized sink for dimethylsulfide.
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DOI：
10.3389/fmicb.2022.950460
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
由于底栖微生物、大型藻类和盐沼植被产生二甲基磺基丙酸酯（DMSP），盐沼是有机硫化合物循环的热点。 DMSP 的降解是二甲硫醚 (DMS) 的来源，二甲硫醚是形成二次有机气溶胶的重要前体。降解 DMS 的微生物在控制可排放到大气中的 DMS 量方面发挥着重要作用。先前的研究表明沉积物微生物种群是 DMS 的主要汇。在这里，我们发现 Sporobolus anglicus（以前称为 Spartina anglica）是一种广泛分布的盐沼植物，被 DMS 降解微生物定殖。通过气相色谱和 13C-DMS 稳定同位素探测评估二甲硫醚的降解潜力，通过 16S rRNA 基因扩增子的高通量测序、甲硫醇氧化酶基因的克隆和测序以及叶际微生物群落的宏基因组分析评估叶际和根际样品中的微生物群落多样性和功能遗传潜力。从叶际和根际样品中回收的微生物群落的 DMS 降解潜力相似。通过 13C-DMS 稳定同位素探测鉴定出活性 DMS 降解剂，其中包括根际样品中与甲基噬菌体和其他鱼立克次体科相关的种群。叶圈中的 DMS 降解者包括黄单胞菌科 (Xanthomonadaceae) 和卤硫杆菌科 (Halothiobacillaceae)。沉积物样品中甲硫醇氧化酶 (mtoX) 基因（DMS 和 DMSP 降解过程中甲硫醇代谢的标志物）的多样性与之前检测到的盐沼 mtoX（包括甲基噬菌体和甲基球菌科的基因）相似。叶际 mtoX 基因与沉积物 mtoX 不同，并且不包括培养细菌的近亲。与水稻、大豆、三叶草和拟南芥等模型植物相比，S. anglicus 叶圈中的微生物多样性明显不同，并且显示出 Gammaproteobacteria 而非 Alphaproteobacteria 占主导地位。微生物 DMS 在 Sporobolus anglicus 叶际和根际降解的潜力表明，盐沼中的 DMS 循环比以前认识的更为复杂，需要对地上活动如何影响 DMS 通量进行更详细的评估。
Saltmarshes are hotspots of organosulfur compound cycling due to production of dimethylsulfoniopropionate (DMSP) by benthic microorganisms, macroalgae, and saltmarsh vegetation. Degradation of DMSP is a source of dimethylsulfide (DMS), an important precursor for formation of secondary organic aerosol. Microorganisms degrading DMS play a role in controlling the amount of DMS available for emission into the atmosphere. Previous work has implicated sediment microbial populations as a major sink for DMS. Here, we show that Sporobolus anglicus (previously known as Spartina anglica), a widely distributed saltmarsh plant, is colonized by DMS-degrading microorganisms. Dimethylsulfide degradation potential was assessed by gas chromatography and 13C-DMS stable isotope probing, microbial community diversity and functional genetic potential in phyllosphere and rhizosphere samples was assessed by high-throughput sequencing of 16S rRNA gene amplicons, cloning and sequencing of methanethiol oxidase genes, and by metagenomic analysis of phyllosphere microbial communities. The DMS degradation potential of microbial communities recovered from phyllosphere and rhizosphere samples was similar. Active DMS-degraders were identified by 13C-DMS stable isotope probing and included populations related to Methylophaga and other Piscirickettsiaceae in rhizosphere samples. DMS-degraders in the phyllosphere included Xanthomonadaceae and Halothiobacillaceae. The diversity in sediment samples of the methanethiol oxidase (mtoX) gene, a marker for metabolism of methanethiol during DMS and DMSP degradation, was similar to previously detected saltmarsh mtoX, including those of Methylophaga and Methylococcaeae. Phyllosphere mtoX genes were distinct from sediment mtoX and did not include close relatives of cultivated bacteria. Microbial diversity in the phyllosphere of S. anglicus was distinct compared to those of model plants such as rice, soybean, clover and Arabidopsis and showed a dominance of Gammaproteobacteria rather than Alphaproteobacteria. The potential for microbial DMS degradation in the phyllosphere and rhizosphere of Sporobolus anglicus suggest that DMS cycling in saltmarshes is more complex than previously recognised and calls for a more detailed assessment of how aboveground activities affect fluxes of DMS.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1038/ismej.2015.37
发表时间：
2015-11
期刊：
The ISME journal
影响因子：
--
作者：
Eyice Ö;Namura M;Chen Y;Mead A;Samavedam S;Schäfer H
通讯作者：
Schäfer H
影响因子：
14.9
作者：
Benson DA;Cavanaugh M;Clark K;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
影响因子：
2.3
作者：
DeBose, Jennifer L.;Nevitt, Gabrielle A.
通讯作者：
Nevitt, Gabrielle A.
影响因子：
4.4
作者：
Ansede, JH;Friedman, R;Yoch, DC
通讯作者：
Yoch, DC