Characterization of lipids from human brain tissues by multinuclear magnetic resonance spectroscopy.
Characterization of lipids from human brain tissues by multinuclear magnetic resonance spectroscopy.
复制标题
通过多核磁共振波谱表征人脑组织的脂质。
作者：
V. Tugnoli;M. Tosi;A. Tinti;A. Trinchero;G. Bottura;G. Fini
多核 ((1)H、(13)C 和 (31)P) 磁共振波谱应用于健康和肿瘤人脑组织总脂质部分的生化表征。检查来自正常大脑、胶质母细胞瘤、间变性少突胶质细胞瘤、少突胶质细胞瘤和脑膜瘤的脂质提取物。此外，还分析了邻近脑膜瘤的囊肿的未知液体含量。直接通过 (1)H-NMR 研究来自胶质母细胞瘤的两份活检样本，无需任何处理（离体 NMR）。 (1)H-和(13)C-NMR 分析可以全面表征脑组织的脂质成分。特别是，高级别肿瘤提取物中胆固醇酯和甘油三酯的存在与血管增殖程度相关，这与正常脑组织和低级别肿瘤不同。 (31)P 光谱显示磷脂酰胆碱是主要的磷脂，其相对含量在神经胶质瘤中较高，与肿瘤细胞的低分化程度和膜更新的改变相关。胶质母细胞瘤样品的离体 (1)H-NMR 数据显示存在与细胞坏死现象相关的移动脂质。我们的数据允许核磁共振获得的生化结果与组织病理学因素（血管和细胞增殖、分化和坏死）之间存在直接关联，这些因素在确定脑肿瘤分级中很重要。
Multinuclear ((1)H, (13)C, and (31)P) magnetic resonance spectroscopy are applied to the biochemical characterization of the total lipid fraction of healthy and neoplastic human brain tissues. Lipid extracts from normal brains, glioblastomas, anaplastic oligodendrogliomas, oligodendrogliomas, and meningiomas are examined. Moreover, the unknown liquid content of a cyst adjacent to a meningioma is analyzed. Two biopsies from glioblastomas are directly studied by (1)H-NMR without any treatment (ex vivo NMR). The (1)H- and (13)C-NMR analysis allows full characterization of the lipid component of the cerebral tissues. In particular, the presence of cholesteryl esters and triglycerides in the extracts of high grade tumors is correlated to the vascular proliferation degree, which is different from normal brain tissue and low grade neoplasms. The (31)P spectra show that phosphatidylcholine is the prominent phospholipid and its relative amount, which is higher in gliomas, is correlated to the low grade of differentiation of tumor cells and an altered membrane turnover. The ex vivo (1)H-NMR data on the glioblastoma samples show the presence of mobile lipids that are correlated to cell necrotic phenomena. Our data allow a direct correlation between biochemical results obtained by NMR and the histopathological factors (vascular and cell proliferations, differentiation, and necrosis) that are prominent in determining brain tumor grading.
通过 1 H-NMR 表征受刺激的人淋巴细胞中的脂质成分。
DOI：
10.1016/0167-4889(90)90241-5
发表时间：
1990
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Sze,DY;Jardetzky,O
通讯作者：
Jardetzky,O