MiR-29a Regulates Radiosensitivity in Human Intestinal Cells by Targeting PTEN Gene
MiR-29a Regulates Radiosensitivity in Human Intestinal Cells by Targeting PTEN Gene
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MiR-29a 通过靶向 PTEN 基因调节人肠细胞的放射敏感性
DOI：
10.1667/rr14428.1
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发表时间：
2016-08
影响因子：
3.4
通讯作者：
Shao, Chunlin
中科院分区：
文献类型：
--
作者：
Yuan, Dexiao;Guo, Fei;Zhou, Cuiping;Shao, Chunlin
结肠直肠癌（CRC）放射治疗期间遇到的两个主要挑战是肿瘤细胞的放射抗性和对正常细胞的损伤。对CRC放射抵抗机制的理解可能会导致克服情感性临床治疗障碍的新策略。在这项研究中，比较了四种细胞系中的miR-29 a表达：正常人肠上皮隐窝细胞系HIEC和三种CRC细胞系HT 29、DLD-1和HCT 116。然后研究miR-29 a在调节细胞放射敏感性中的作用。这项研究的结果表明，miR-29 a模拟物增强了具有低水平内在miR-29 a的HIEC、HT 29和DLD-1细胞的辐射抗性。另一方面，miR-29 a抑制剂在照射后显著致敏具有高水平miR-29 a的HCT 116细胞。进一步的研究表明，PTEN是miR-29 a的直接功能靶点，参与了放射敏感性的调节。MiR-29 a可通过负调控PTEN表达激活PI 3 K/Akt信号通路。结论：miR-29 a可能通过靶向PTEN基因调节大肠癌细胞的放射敏感性，提示miR-29 a可能是一种提高大肠癌放射敏感性的新途径。
Two major challenges encountered during radiotherapy for colorectal cancer (CRC) are radioresistance of tumor cells and damage to normal cells. An understanding of the mechanisms of radioresistance in CRC may lead to new strategies for overcoming obstacles to affective clinical therapy. In this study, the miR-29a expression was compared among four cell lines: the normal human intestinal epithelial crypt cell line, HIEC and three CRC cell lines, HT29, DLD-1 and HCT116. The roles of miR-29a in regulating cellular radiosensitivity were then investigated. The findings from this study showed that miR-29a mimic enhanced radioresistance in HIEC, HT29 and DLD-1 cells with low levels of intrinsic miR-29a. On the other hand, a miR-29a inhibitor significantly sensitized HCT116 cells with high levels of miR-29a after irradiation. Further studies indicated that PTEN was the direct functional target of miR-29a and was involved in radiosensitivity. MiR-29a could activate the PI3K/Akt signaling pathway through negatively regulated PTEN expression. In conclusion, miR-29a may regulate the radiosensitivity of intestinal cell lines by targeting the PTEN gene, which indicates miR-29a might serve as a novel approach to enhance radiosensitivity in CRC.
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miRNA在肿瘤放射敏感性中的调控机制和临床前景。
影响因子：
4.7
作者：
Zhao L;Bode AM;Cao Y;Dong Z
通讯作者：
Dong Z
DOI：
10.1084/jem.20090831
发表时间：
2010-03-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Han YC;Park CY;Bhagat G;Zhang J;Wang Y;Fan JB;Liu M;Zou Y;Weissman IL;Gu H
通讯作者：
Gu H
DOI：
10.1186/1748-717x-7-195
发表时间：
2012-11-20
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Svoboda M;Sana J;Fabian P;Kocakova I;Gombosova J;Nekvindova J;Radova L;Vyzula R;Slaby O
通讯作者：
Slaby O
MicroRNA-221和microRNA-222通过靶向PTEN调节胃癌细胞增殖和放射抗性。
影响因子：
3.8
作者：
Chun-Zhi Z;Lei H;An-Ling Z;Yan-Chao F;Xiao Y;Guang-Xiu W;Zhi-Fan J;Pei-Yu P;Qing-Yu Z;Chun-Sheng K
通讯作者：
Chun-Sheng K
影响因子：
--
作者：
Ma Y;Xia H;Liu Y;Li M
通讯作者：
Li M