Apoptosis in the medaka embryo in the early developmental stage.

Apoptosis in the medaka embryo in the early developmental stage.
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DOI:
10.1267/ahc.06013
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发表时间:
2007-02-27
影响因子:
2.4
通讯作者:
Yokoyama T
Yokoyama T
中科院分区:
生物学4区
文献类型:
--
作者:
Iijima N;Yokoyama T

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细胞凋亡是各种器官发育过程中的重要事件。本研究利用原位末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记技术,对青竹胚胎发育过程中细胞凋亡的时空分布进行了可视化研究,为发育生物学和遗传学研究提供了一种有用的模型。凋亡细胞主要分布在中枢神经系统和尾芽。在大脑和视网膜中,大部分细胞凋亡发生在限制期。与caspase3A和caspase3B的原位杂交结果表明,它们分别分布在尾芽和头部。这些结果表明,两种类型的caspase3参与了不同区域的细胞凋亡。
Apoptosis is an important event of the development of various organs. In this study, we used in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) to visualize the temporal and spatial distribution of apoptosis in the developing medaka embryo, which is a useful model for developmental biology and genetics. Most of the apoptotic cells were distributed in the central nervous system and tailbud. In the brain and retina, most of the apoptosis occurred in the restricted period. In situ hybridization against caspase 3A and caspase 3B showed that these were distributed in the tailbud and the head, respectively. These results suggested that two types of caspase 3 were involved in apoptosis in different areas.
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发表时间: 2001-11-15
影响因子: 4.1
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