Interleukin‐9 receptor gene is transcriptionally regulated by nucleolin in T‐Cell lymphoma cells
Interleukin‐9 receptor gene is transcriptionally regulated by nucleolin in T‐Cell lymphoma cells
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T 细胞淋巴瘤细胞中白细胞介素 9 受体基因的转录受核仁素调控
DOI：
10.1002/mc.20834
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发表时间：
2012
影响因子：
4.6
通讯作者：
Y. Shimada
中科院分区：
文献类型：
--
作者：
Y. Shang;S. Kakinuma;M. Nishimura;Yoshiro Kobayashi;K. Nagata;Y. Shimada
白细胞介素-9（IL-9）是一种多功能细胞因子，不仅在免疫和炎症反应中起作用，而且在多种转化细胞系中参与促生长和抗凋亡活性，这表明在肿瘤发生中具有潜在作用。IL-9受体（IL-9 R）的过度表达发生在几种类型的人类白血病和辐射诱导的小鼠T细胞淋巴瘤（TL）中。然而，在白血病发生过程中调节IL-9 R基因（Il 9 r）转录的分子机制尚未完全了解。使用小鼠TL细胞系，具有高表达的IL 9 r，我们试图剖析其启动子结构。在这里，我们发现Il 9 r的活性启动子位于5′-侧翼富含AT的区域。染色质免疫沉淀显示，在体内启动子区的染色质结构的开放与核仁素结合偶联。免疫组织化学分析证实核仁素在TL细胞核中的定位增加。这些数据表明，IL 9 r的表达增加与TL细胞中IL 9 r的5′-侧翼区域中富含AT的区域的核仁素结合增加以及染色质开放相关。© 2011 Wiley Periodicals，Inc.
Interleukin‐9 (IL‐9) is a multifunctional cytokine that not only has roles in immune and inflammatory responses but also is involved in growth‐promoting and anti‐apoptotic activities in multiple transformed cell lines, which suggests a potential role in tumorigenesis. Over‐expression of the receptor of IL‐9 (IL‐9R) occurs in several types of human leukemias and in radiation‐induced mouse T‐cell lymphoma (TL). The molecular mechanism that regulates transcription of the IL‐9R gene (Il9r) during leukemogenesis is, however, not well understood. Using a mouse TL cell line that has high expression of Il9r, we sought to dissect its promoter structure. Here we show that the active promoter for Il9r is located in the 5′‐flanking AT‐rich region. Chromatin immunoprecipitation showed the opening of chromatin structure of the promoter region coupled with nucleolin binding in vivo. Immunohistochemical analysis confirmed the increased localization of nucleolin in the nuclei of TL cells. These data indicate that increased expression of Il9r is associated with an increased binding of nucleolin, coupled with chromatin opening, to an AT‐rich region in the 5′‐flanking region of Il9r in TL cells. © 2011 Wiley Periodicals, Inc.
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影响因子：
10.1
作者：
J. Barata;T. D. Keenan;A. Silva;L. Nadler;V. Boussiotis;A. Cardoso
通讯作者：
J. Barata;T. D. Keenan;A. Silva;L. Nadler;V. Boussiotis;A. Cardoso
影响因子：
20.3
作者：
Otake, Yoko;Soundararajan, Sridharan;Fernandes, Daniel J.
通讯作者：
Fernandes, Daniel J.
影响因子：
32.4
作者：
Kim, J;Sif, S;Georgopoulos, K
通讯作者：
Georgopoulos, K
在正常和癌性乳腺上皮细胞中鉴定具有短 5-UTR 的新型 RARbeta2 转录物变体。
DOI：
10.1038/sj.onc.1208284
发表时间：
2005
期刊：
Oncogene.
影响因子：
--
作者：
Peng,Xinjian;Mehta,RajendraG;Tonetti,DebraA;Christov,Konstantin
通讯作者：
Christov,Konstantin