TMEM65 is a mitochondrial inner-membrane protein.
TMEM65 is a mitochondrial inner-membrane protein.
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作者：
Nishimura N;Gotoh T;Oike Y;Yano M
已有研究表明，TMEM65的表达受类固醇受体RNA激活剂(SRA)的调节。然而，到目前为止，TMEM65的定位和功能仍不清楚。我们分析了TMEM65在细胞内的定位。免疫印迹和免疫染色分析显示TMEM65的线粒体定位。碱提取分析和洋地黄素提取试验表明，TMEM65是一种完整的膜蛋白，定位于线粒体内膜。利用TMEM65的缺失突变体分析表明，该蛋白的N-末端(1-20)具有足够的线粒体靶向性，并且该线粒体靶向信号被切割在氨基酸残基35和之间，其中含有一个可能的基质加工蛋白酶的识别位点。综上所述，这些结果表明TMEM65被导入线粒体，整合到线粒体内膜，并通过MPP加工成成熟的形式。
It has been reported that the expression of TMEM65 is regulated by steroid receptor RNA activator (SRA). To date, however, the localization and function of TMEM65 remained unknown. We analyzed the intracellular localization of TMEM65. Immunoblot and immunostaining analysis revealed mitochondrial localization of TMEM65. Alkali extraction analysis and digitonin extraction test using isolated mitochondria revealed that TMEM65 is an integral membrane protein that localizes to the inner-membrane of mitochondria. Analysis using deletion mutants of TMEM65 suggested that the N-terminal region (1–20) of this protein is sufficient for mitochondrial targeting and that this mitochondrial targeting signal (MTS) is cleaved between the amino acid positions 35 and 64, which contain a putative recognition site of matrix processing protease (MPP). Together, these results suggest that TMEM65 is imported into the mitochondria, integrated into mitochondrial inner-membrane, and processed into its mature form by an MPP.
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影响因子：
3.3
作者：
Sasarman F;Brunel-Guitton C;Antonicka H;Wai T;Shoubridge EA;LSFC Consortium
通讯作者：
LSFC Consortium
影响因子：
5.3
作者：
Yano, M;Hoogenraad, N;Mori, M
通讯作者：
Mori, M
线粒体：在疾病和健康中。
影响因子：
64.5
作者：
Nunnari J;Suomalainen A
通讯作者：
Suomalainen A
DOI：
10.1210/me.2009-0427
发表时间：
2010-05-01
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Foulds, Charles E;Tsimelzon, Anna;O'Malley, Bert W
通讯作者：
O'Malley, Bert W
DOI：
10.1073/pnas.242716699
发表时间：
2003-01-21
影响因子：
11.1
作者：
Mootha, VK;Lepage, P;Lander, ES
通讯作者：
Lander, ES