ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis.
ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis.
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DOI：
10.1038/s41467-018-07225-7
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发表时间：
2018-11-14
影响因子：
16.6
通讯作者：
Hill C
中科院分区：
文献类型：
--
作者：
Shkoporov AN;Khokhlova EV;Fitzgerald CB;Stockdale SR;Draper LA;Ross RP;Hill C
噬菌体是广泛且普遍存在的有尾噬菌体家族，预测感染拟杆菌目的细菌。尽管在约50%的个体中发现，并代表高达90%的人类肠道病毒组，但该病毒家族的成员从未在培养物中分离出来，并且仍然研究不足。在这里，我们报告了从人类粪便材料中分离出的CrAs001。这种噬菌体感染了人类肠道共生体拟杆菌，证实了先前对可能宿主的计算机预测。DNA测序表明，噬菌体基因组是圆形的，大小为102 kb，并具有不寻常的结构特征。此外，电子显微镜证实，ΦcrAss001具有足状病毒样形态。尽管没有明显的溶原性基因，但ΦcrAss001以不破坏宿主细菌增殖的方式复制，并且能够在连续宿主培养物中维持数周。噬菌体家族的噬菌体尚未被分离，尽管在人类肠道中高度丰富。在这里，Shkoporov等人在纯培养物中分离出其中一种病毒，并表明它感染了人类肠道共生体拟杆菌。
CrAssphages are an extensive and ubiquitous family of tailed bacteriophages, predicted to infect bacteria of the order Bacteroidales. Despite being found in ~50% of individuals and representing up to 90% of human gut viromes, members of this viral family have never been isolated in culture and remain understudied. Here, we report the isolation of a CrAssphage (ΦCrAss001) from human faecal material. This bacteriophage infects the human gut symbiont Bacteroides intestinalis, confirming previous in silico predictions of the likely host. DNA sequencing demonstrates that the bacteriophage genome is circular, 102 kb in size, and has unusual structural traits. In addition, electron microscopy confirms that ΦcrAss001 has a podovirus-like morphology. Despite the absence of obvious lysogeny genes, ΦcrAss001 replicates in a way that does not disrupt proliferation of the host bacterium, and is able to maintain itself in continuous host culture during several weeks. Bacteriophages of the crAssphage family have not yet been isolated, despite being highly abundant in the human gut. Here, Shkoporov et al. isolate in pure culture one of these viruses and show that it infects the human gut symbiont Bacteroides intestinalis.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1305923110
发表时间：
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DOI：
10.1073/pnas.1305956110
发表时间：
2013-07-30
影响因子：
11.1
作者：
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影响因子：
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作者：
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通讯作者：
Pevzner PA