Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging Published, JLR Papers in Press, November 17, 2007.
Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging Published, JLR Papers in Press, November 17, 2007.
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通过簇飞行时间二次离子质谱成像绘制人类营养不良肌肉中的脂质图已出版，JLR Papers in Press，2007 年 11 月 17 日。
DOI：
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发表时间：
2008
影响因子：
6.5
通讯作者：
O. Laprévote
中科院分区：
文献类型：
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作者：
Nora Tahallah;A. Brunelle;S. de la Porte;O. Laprévote
来自男性对照和杜氏肌营养不良症受影响的儿童的人横纹肌样品在静态西姆斯条件下使用25 keV Bi 3+液体金属离子枪进行簇-飞行时间-二次离子质谱（cluster-ToF-西姆斯）成像。在500 × 500 μm2（图像分辨率，256 × 256像素）的几个区域内，以正离子和负离子模式采集光谱和离子密度图或二次离子图像。观察到各种脂质的特征性分布。维生素E和磷脂酰肌醇被发现集中在细胞内，而完整的磷酸胆碱积累在最受损的区域的营养不良的肌肉，连同胆固醇和鞘磷脂物种。脂肪酰基链组成根据区域而变化，从而可以估计局部损伤程度。
Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging using a 25 keV Bi3+ liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 × 500 μm2 (image resolution, 256 × 256 pixels). Characteristic distributions of various lipids were observed. Vitamin E and phosphatidylinositols were found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chain composition varied depending on the region, allowing estimation of the local damage extent.
影响因子：
16.6
作者：
Wiseman, JM;Puolitaival, SM;Caprioli, RM
通讯作者：
Caprioli, RM
影响因子：
15
作者：
Monroe, EB;Jurchen, JC;Sweedler, JV
通讯作者：
Sweedler, JV