Benefit of a single simulated hypobaric hypoxia in healthy mice performance and analysis of mitochondria-related gene changes.
Benefit of a single simulated hypobaric hypoxia in healthy mice performance and analysis of mitochondria-related gene changes.
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DOI：
10.1038/s41598-020-80425-8
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发表时间：
2021-02-24
影响因子：
4.6
通讯作者：
Wang YY
中科院分区：
文献类型：
--
作者：
Wu FF;Zhang KL;Wang ZM;Yang Y;Li SH;Wang JQ;Ma J;Yang YL;Zhang HF;Wang YY
模拟低压缺氧（SHH）训练已被用来提高跑步表现。然而，尚无研究评估单次SHH暴露对健康小鼠表现的影响，并分析中枢神经系统中线粒体相关基因的变化。本研究使用小鼠减压室模拟5000米高海拔轻度低压缺氧或8000米重度低压缺氧16小时（分别为SHH5000和SHH8000）。然后，通过改进的 Noldus 视频跟踪记录小鼠行为测试。第三，在小脑、海马和腓肠肌中评估了 SHH 对 Drp1、Mfn1、Mfn2、Opa1、TFAM、SGK1、UCP2 和 UCP4 8 个线粒体相关基因的影响。结果表明，一次轻度或重度 HH 可以改善健康小鼠的表现。在小脑中，所有 8 个检测到的基因中有 6 个（Mfn2 和 UCP4 除外）在 SHH 后没有变化。在海马体中，所有检测到的基因在 SHH 后都没有改变。在肌肉中，所有 8 个检测到的基因中有 7 个（Opa1 除外）在 SHH 后没有改变。目前的研究表明，单一 SHH 对健康小鼠的表现有好处，这归因于稳定的线粒体对抗轻度应激状态。
Simulated hypobaric hypoxia (SHH) training has been used to enhance running performance. However, no studies have evaluated the effects of a single SHH exposure on healthy mice performance and analyzed the changes of mitochondria-related genes in the central nervous system. The current study used a mouse decompression chamber to simulate mild hypobaric hypoxia at the high altitude of 5000 m or severe hypobaric hypoxia at 8000 m for 16 h (SHH5000 & SHH8000, respectively). Then, the mouse behavioral tests were recorded by a modified Noldus video tracking. Third, the effects of SHH on 8 mitochondria-related genes of Drp1, Mfn1, Mfn2, Opa1, TFAM, SGK1, UCP2 and UCP4, were assessed in cerebellum, hippocampus and gastrocnemius muscles. The results have shown that a single mild or severe HH improves healthy mice performance. In cerebellum, 6 of all 8 detected genes (except Mfn2 and UCP4) did not change after SHH. In hippocampus, all detected genes did not change after SHH. In muscles, 7 of all 8 detected genes (except Opa1) did not change after SHH. The present study has indicated the benefit of a single SHH in healthy mice performance, which would due to the stabilized mitochondria against a mild stress state.
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影响因子：
7.3
作者：
Chitra, Loganathan;Boopathy, Rathanam
通讯作者：
Boopathy, Rathanam
影响因子：
8.1
作者：
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DOI：
10.1055/s-2002-25080
发表时间：
2002-04-01
影响因子：
0.4
作者：
Jürgens, KD;Gros, G
通讯作者：
Gros, G
影响因子：
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