Defining the morphological phenotype: 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells
Defining the morphological phenotype: 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells
复制标题
定义形态表型：2β,3β-环核苷酸 3β-磷酸二酯酶 (CNPase) 是一种用于原位检测犬而非大鼠嗅鞘细胞的新型标记物
DOI：
10.1007/s00441-011-1168-8
复制
发表时间：
2011
影响因子：
3.6
通讯作者：
K Wewetzer
中科院分区：
文献类型：
--
作者：
Omar M;Bock P;Kreutzer R;Ziege S;Imbschweiler I;Hansmann F;Peck CT;Baumgärtner W;K Wewetzer
嗅鞘细胞（Olfactory ensheathing cells，OECs）是嗅神经和嗅球的非髓鞘神经胶质细胞。在正常发育过程中原位OEC的零碎表征可能是由于它们的尺寸小，需要复杂的超微结构分析，以及用于原位检测的可用标记物仅由OEC亚群表达或在发育过程中丢失的事实。在本研究中，我们寻找在嗅鞘细胞中稳定表达的标记物，并与原型标记物p75 NTR相比，研究了CNR（一种早期少突胶质细胞/雪旺细胞标记物）的时空分布。抗CNE 2抗体标记犬嗅鞘细胞，但不原位大鼠，而雪旺细胞和少突胶质细胞在这两个物种呈阳性。在狗的CNR免疫反应性仅限于所有的嗅鞘细胞在整个出生后的发展和整个细胞体，包括其最精细的过程，而p75 NTR主要是在神经周细胞和只在一些新生儿嗅鞘细胞检测。成人嗅球切片显示CNR表达后4和10天，而p75 NTR是检测到后10天在体外。最后，用成纤维细胞生长因子-2处理纯化的成年犬嗅鞘细胞，在蛋白和mRNA水平上显著降低了CNE 1的表达。综上所述，我们得出结论，CNR，而不是p75 NTR是一个稳定的标记，适合于在原位可视化的OEC，这将有利于他们的光显微镜表征和挑战我们的一般观点的OEC标记表达原位。犬而非大鼠的嗅鞘细胞表达CNY的事实支持了来自大型动物的神经胶质与啮齿动物有实质性差异的观点。
Olfactory ensheathing cells (OECs) are the non-myelinating glial cells of the olfactory nerves and bulb. The fragmentary characterization of OECs in situ during normal development may be due to their small size requiring intricate ultrastructural analysis and to the fact that available markers for in situ detection are either expressed only by OEC subpopulations or lost during development. In the present study, we searched for markers with stable expression in OECs and investigated the spatiotemporal distribution of CNPase, an early oligodendrocyte/Schwann cell marker, in comparison with the prototype marker p75NTR. Anti-CNPase antibodies labeled canine but not rat OECs in situ, while Schwann cells and oligodendrocytes were positive in both species. CNPase immunoreactivity in the dog was confined to all OECs throughout the postnatal development and associated with the entire cell body, including its finest processes, while p75NTRwas mainly detected in perineural cells and only in some neonatal OECs. Adult olfactory bulb slices displayed CNPase expression after 4 and 10 days, while p75NTRwas detectable only after 10 days in vitro. Finally, treatment of purified adult canine OECs with fibroblast growth factor-2 significantly reduced CNPase expression at the protein and mRNA level. Taken together, we conclude that CNPase but not p75NTRis a stable marker suitable for in situ visualization of OECs that will facilitate their light-microscopic characterization and challenge our general view of OEC marker expression in situ. The fact that canine but not rat OECs expressed CNPase supports the idea that glia from large animals differs substantially from rodents.
登录
查看更多内容
轴突信号传导和嗅觉鞘细胞的形成：一个假设。
影响因子：
--
作者：
K. Wewetzer;G. Brandes
通讯作者：
G. Brandes
胚胎猴端脑中低亲和力 p75 神经生长因子受体表达：不同细胞元件中的时间和定位
影响因子：
3.3
作者：
D. Meinecke;P. Rakić
通讯作者：
P. Rakić
雪旺细胞和肠神经胶质细胞对微环境的表型可塑性。
DOI：
--
发表时间：
1991
影响因子：
11.1
作者：
C. Dulac;N. L. Le Douarin
通讯作者：
N. L. Le Douarin
消减杂交鉴定嗅鞘细胞和神经干细胞差异表达的基因
DOI：
10.1016/j.ijdevneu.2009.08.019
发表时间：
2010
影响因子：
1.8
作者：
A. Rojas;N. M. Torres;G. Gudiño;Daniel Ortuño
通讯作者：
Daniel Ortuño
影响因子：
5.3
作者：
Kreutzer R;Kreutzer M;Pröpsting MJ;Sewell AC;Leeb T;Naim HY;Baumgärtner W
通讯作者：
Baumgärtner W