Neutrophils are essential as a source of IL-17 in the effector phase of arthritis.
Neutrophils are essential as a source of IL-17 in the effector phase of arthritis.
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DOI：
10.1371/journal.pone.0062231
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mimori T
中科院分区：
文献类型：
--
作者：
Katayama M;Ohmura K;Yukawa N;Terao C;Hashimoto M;Yoshifuji H;Kawabata D;Fujii T;Iwakura Y;Mimori T
Th17已被证明在关节炎的发展过程中起着关键作用。然而，IL-17在T细胞非依赖性效应阶段的作用还没有被充分研究。我们采用K/BxN血清诱导的关节炎模型，研究了IL-17是否参与了关节炎的效应期。将K/BxN血清转移到IL-17基因敲除(KO)小鼠、SCID小鼠及其对照小鼠，并随时间评估关节炎。为了弄清IL-17在效应期的来源，从IL-17 KO或对照小鼠采集中性粒细胞或CD4+T细胞，与K/BxN血清一起注射到IL-17 KO受体小鼠体内。为了检测中性粒细胞在刺激时是否分泌IL-17，在 体外用免疫复合物刺激中性粒细胞，用ELISA法测定培养上清液中IL-17的含量。K/BxN血清诱导的关节炎在IL-17KO小鼠中比在WT小鼠中要轻得多。由于K/BxN血清转移的SCID小鼠出现了严重的关节炎，血清IL-17浓度较高，我们推测中性粒细胞是IL-17的主要来源。当野生型(WT)而非IL-17 KO中性粒细胞与K/BxN血清共同注射给IL-17 KO小鼠时，关节炎加重，而联合注射野生型(WT)CD4+T细胞则无作用。在体外，免疫复合体刺激中性粒细胞可引起IL-17的分泌。在关节炎的效应期，中性粒细胞是IL-17的重要来源。中性粒细胞分泌IL-17的触发因素可能是免疫复合体。
Th17 has been shown to have a pivotal role in the development of arthritis. However, the role of IL-17 in the T cell-independent effector phase has not fully been examined. We investigated whether IL-17 is involved in the effector phase of arthritis by using K/BxN serum-induced arthritis model. K/BxN serum was transferred into IL-17 knockout (KO) mice, SCID mice and their control mice, and arthritis was evaluated over time. In order to clarify the source of IL-17 in the effector phase, neutrophils or CD4+ T cells collected from IL-17 KO or control mice were injected into IL-17 KO recipient mice together with K/BxN serum. To examine if neutrophils secrete IL-17 upon stimulation, neutrophils were stimulated with immune complex in vitro and IL-17 in the supernatant was measured by ELISA. K/BxN serum-induced arthritis was much less severe in IL-17 KO mice than in WT mice. Since K/BxN serum-transferred SCID mice developed severe arthritis with high serum IL-17 concentration, we speculated neutrophils are the responsible player as an IL-17 source. When wild type (WT) but not IL-17 KO neutrophils were co-injected with K/BxN serum into IL-17 KO mice, arthritis was exacerbated, whereas co-injection of WT CD4+ T cells had no effect. In vitro, stimulation of neutrophils with immune complexcaused IL-17 secretion. Neutrophils are essential as a source of IL-17 in the effector phase of arthritis. The trigger of secreting IL-17 from neutrophils may be immune complex.
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DOI：
10.4049/jimmunol.1100123
发表时间：
2011-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lin AM;Rubin CJ;Khandpur R;Wang JY;Riblett M;Yalavarthi S;Villanueva EC;Shah P;Kaplan MJ;Bruce AT
通讯作者：
Bruce AT
影响因子：
32.4
作者：
Yao, ZB;Fanslow, WC;Spriggs, MK
通讯作者：
Spriggs, MK
影响因子：
4.4
作者：
Hueber, Axel J.;Asquith, Darren L.;McInnes, Iain B.
通讯作者：
McInnes, Iain B.
影响因子：
56.9
作者：
Lee, DM;Friend, DS;Brenner, MB
通讯作者：
Brenner, MB
DOI：
10.1084/jem.183.6.2593
发表时间：
1996-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Fossiez F;Djossou O;Chomarat P;Flores-Romo L;Ait-Yahia S;Maat C;Pin JJ;Garrone P;Garcia E;Saeland S;Blanchard D;Gaillard C;Das Mahapatra B;Rouvier E;Golstein P;Banchereau J;Lebecque S
通讯作者：
Lebecque S