Changes in microRNA expression profile in hippocampus during the acquisition and extinction of cocaine-induced conditioned place preference in rats.
Changes in microRNA expression profile in hippocampus during the acquisition and extinction of cocaine-induced conditioned place preference in rats.
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大鼠可卡因诱导的条件性位置偏好的获得和消失过程中海马 microRNA 表达谱的变化，
DOI：
10.1186/1423-0127-20-96
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发表时间：
2013-12-20
影响因子：
11
通讯作者：
Guan X
中科院分区：
文献类型：
--
作者：
Chen CL;Liu H;Guan X
MicroRNA（miRNA）是药物滥用的重要参与者。然而，它们在可卡因滥用的神经系统疾病中的表达谱尚未得到很好的表征。在这里，我们探讨了重复可卡因暴露和随后的可卡因治疗戒断后大鼠海马中miRNA表达的变化。采用条件性位置偏爱（CPP）程序研究大鼠可卡因觅药行为的获得和消退。采用微阵列技术检测大鼠海马组织中miRNAs的表达水平。定量RT-PCR进一步证实了微阵列研究的结果。最后，通过生物信息学方法预测可卡因反应性miRNA的潜在靶基因。miRNA芯片检测发现，与正常对照组相比，可卡因诱导的CPP在大鼠海马中有34个miRNA水平发生变化，可卡因诱导的CPP消失后有42个miRNA水平发生变化。来自qRT-PCR研究的发现支持来自微阵列分析的结果。目前的研究表明，在可卡因诱导的CPP的获得和消退过程中，大鼠海马中miRNA表达的动态变化。一些先前已报道参与脑部疾病和药物滥用的miRNA，包括miR-133 b、miR-134、miR-181 c、miR-191、miR-22、miR-26 b、miR-382、miR-409- 3 p和miR-504，发现在重复可卡因暴露和随后戒除可卡因治疗后其表达发生变化。这些发现可能会扩展我们对可卡因滥用的监管网络的理解，并可能为未来治疗药物滥用提供新的靶点。
MicroRNA (miRNA) emerges as important player in drug abuse. Yet, their expression profile in neurological disorder of cocaine abuse has not been well characterized. Here, we explored the changes of miRNA expression in rat hippocampus following repeated cocaine exposure and subsequent abstinence from cocaine treatment. Conditioned place preference (CPP) procedure was used to assess the acquisition and extinction of cocaine-seeking behavior in rats. MiRNA microarray was performed to examine miRNAs levels in rat hippocampus. Quantitative RT-PCR was conducted to further confirm results in microarray study. Finally, bioinformatic predictions were made to suggest potential target genes of cocaine-responsive miRNA in this study. MiRNA array found that 34 miRNA levels were changed in rat hippocampus while acquiring cocaine CPP and 42 miRNAs levels were altered after the cocaine-induced CPP were extinguished, as compared to normal controls. The findings from qRT-PCR study support results from microarray analysis. The current study demonstrated dynamic changes in miRNA expression in rat hippocampus during the acquisition and extinction of cocaine-induced CPP. Some miRNAs which have been previously reported to be involved in brain disorders and drug abuse, including miR-133b, miR-134, miR-181c, miR-191, miR-22, miR-26b, miR-382, miR-409-3p and miR-504, were found to be changed in their expression following repeated cocaine exposure and subsequent abstinence from cocaine treatment. These findings may extend our understanding of the regulatory network underlying cocaine abuse and may provide new targets for the future treatment of drug abuse.
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影响因子：
25
作者：
Im, Heh-In;Hollander, Jonathan A.;Bali, Purva;Kenny, Paul J.
通讯作者：
Kenny, Paul J.
影响因子：
3.7
作者：
Barreto-Valer K;López-Bellido R;Macho Sánchez-Simón F;Rodríguez RE
通讯作者：
Rodríguez RE
影响因子：
1.7
作者：
Meyers, RA;Zavala, AR;Neisewander, JL
通讯作者：
Neisewander, JL
影响因子：
5.3
作者：
Straiker, AJ;Borden, CR;Sullivan, JM
通讯作者：
Sullivan, JM
影响因子：
3.7
作者：
Caputo V;Sinibaldi L;Fiorentino A;Parisi C;Catalanotto C;Pasini A;Cogoni C;Pizzuti A
通讯作者：
Pizzuti A