Characterization of Protein-Membrane Interactions in Yeast Autophagy.

Characterization of Protein-Membrane Interactions in Yeast Autophagy.
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DOI:
10.3390/cells11121876
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发表时间:
2022-06-09
期刊:
影响因子:
6
通讯作者:
--
中科院分区:
生物学2区
文献类型:
--
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Cells rely on autophagy to degrade cytosolic material and maintain homeostasis. During autophagy, content to be degraded is encapsulated in double membrane vesicles, termed autophagosomes, which fuse with the yeast vacuole for degradation. This conserved cellular process requires the dynamic rearrangement of membranes. As such, the process of autophagy requires many soluble proteins that bind to membranes to restructure, tether, or facilitate lipid transfer between membranes. Here, we review the methods that have been used to investigate membrane binding by the core autophagy machinery and additional accessory proteins involved in autophagy in yeast. We also review the key experiments demonstrating how each autophagy protein was shown to interact with membranes.
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