Substantial Epigenetic Variation Causing Flower Color Chimerism in the Ornamental Tree Prunus mume Revealed by Single Base Resolution Methylome Detection and Transcriptome Sequencing.
Substantial Epigenetic Variation Causing Flower Color Chimerism in the Ornamental Tree Prunus mume Revealed by Single Base Resolution Methylome Detection and Transcriptome Sequencing.
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DOI：
10.3390/ijms19082315
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发表时间：
2018-08-07
影响因子：
5.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Ma KF;Zhang QX;Cheng TR;Yan XL;Pan HT;Wang J
甲基胞嘧啶修饰引起的表观遗传改变参与基因调控和转座因子（TE）抑制，导致表型变异。虽然已经研究了DNA甲基化和TE抑制对花、果实、种皮和叶片色素沉着的影响，但甲基化与花色嵌合的关系尚不清楚。本研究采用比较甲基组学-转录组学方法，探讨了“丹班调枝”李嵌合花形成的分子机制。高效液相色谱-电喷雾电离质谱分析结果表明，白、红两色花瓣组织的差异直接与花青素（花青素3,5- o -二葡萄糖苷、花青素3- o -葡萄糖苷和芍药苷3- o -葡萄糖苷）的积累有关。接下来，我们绘制了首次生成的甲基组，发现11.29-14.83%的基因组胞嘧啶位点被甲基化。我们还发现基因表达总体上与甲基胞嘧啶水平呈负相关，并发现了WT和RT之间显著的表观遗传差异。此外，我们检测了WT和RT之间的差异甲基化区域（DMRs）和dmr相关基因，并得出结论，这些基因中的许多基因，包括差异表达基因（DEGs）和转录因子基因，都是花青素调控途径的关键参与者。重要的是，一些相关的deg含有TE插入，这些插入也被甲基胞嘧啶修饰。上述证据表明，花梅的花色嵌合是由关键基因的DNA甲基化和TEs诱导的。
Epigenetic changes caused by methylcytosine modification participate in gene regulation and transposable element (TE) repression, resulting in phenotypic variation. Although the effects of DNA methylation and TE repression on flower, fruit, seed coat, and leaf pigmentation have been investigated, little is known about the relationship between methylation and flower color chimerism. In this study, we used a comparative methylomic–transcriptomic approach to explore the molecular mechanism responsible for chimeric flowers in Prunus mume “Danban Tiaozhi”. High-performance liquid chromatography-electrospray ionization mass spectrometry revealed that the variation in white (WT) and red (RT) petal tissues in this species is directly due to the accumulation of anthocyanins, i.e., cyanidin 3,5-O-diglucoside, cyanidin 3-O-glucoside, and peonidin 3-O-glucoside. We next mapped the first-ever generated methylomes of P. mume, and found that 11.29–14.83% of the genomic cytosine sites were methylated. We also determined that gene expression was negatively correlated with methylcytosine level in general, and uncovered significant epigenetic variation between WT and RT. Furthermore, we detected differentially methylated regions (DMRs) and DMR-related genes between WT and RT, and concluded that many of these genes, including differentially expressed genes (DEGs) and transcription factor genes, are critical participants in the anthocyanin regulatory pathway. Importantly, some of the associated DEGs harbored TE insertions that were also modified by methylcytosine. The above evidence suggest that flower color chimerism in P. mume is induced by the DNA methylation of critical genes and TEs.
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影响因子：
14.9
作者：
Feng H;Conneely KN;Wu H
通讯作者：
Wu H
影响因子：
6.9
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影响因子：
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作者：
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影响因子：
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