Iontophoretic transport of charged macromolecules across human sclera.
Iontophoretic transport of charged macromolecules across human sclera.
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DOI：
10.1016/j.ijpharm.2009.12.046
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发表时间：
2010-03-30
影响因子：
5.8
通讯作者：
Li, S. Kevin
中科院分区：
文献类型：
--
作者：
Chopra, Poonam;Hao, Jinsong;Li, S. Kevin
离子电渗的作用机制已在兔巩膜中用小分子物质进行了研究。本研究的目的是检查带电大分子通过离体人巩膜的经巩膜离子电渗转运。被动和2毫安离子电渗传输实验进行了并排扩散细胞与人巩膜。本研究测定了离子导入对模型药物贝伐单抗（BEV）和模型药物牛血清白蛋白（BSA）、聚苯乙烯磺酸（PSS）经巩膜转运的影响。四乙基铵（TEA）和水杨酸（SA）的被动和离子电渗转运实验以及大分子的被动转运实验作为对照。离子电渗促进TEA和SA穿过人巩膜的转运的结果与先前的兔巩膜研究中的结果一致。对于大分子BSA和BEV的离子电渗转运，阳极离子电渗中观察到的离子电渗通量高于被动和阴极离子电渗。这表明了电渗透的重要性。对于CIPSS，与阳极离子电渗相比，在阴极离子电渗中观察到更高的离子电渗通量。电渗和电泳影响离子电渗通量的大分子，电渗和电泳的相对贡献是一个函数的分子大小和电荷的大分子。
The mechanisms of transscleral iontophoresis have been investigated previously with small molecules in rabbit sclera. The objective of the present study was to examine transscleral iontophoretic transport of charged macromolecules across excised human sclera. Passive and 2-mA iontophoretic transport experiments were conducted in side-by-side diffusion cells with human sclera. The effects of iontophoresis upon transscleral transport of model permeants bovine serum albumin (BSA) and polystyrene sulfonic acid (PSS) as well as a model drug bevacizumab (BEV) were determined. Passive and iontophoretic transport experiments of tetraethylammonium (TEA) and salicylic acid (SA) and passive transport experiments of the macromolecules served as the controls. The results of iontophoresis enhanced transport of TEA and SA across human sclera were consistent with those in a previous rabbit sclera study. For the iontophoretic transport of macromolecules BSA and BEV, higher iontophoretic fluxes were observed in anodal iontophoresis as compared to passive and cathodal iontophoresis. This suggests the importance of electroosmosis. For the polyelectrolyte PSS, higher iontophoretic flux was observed in cathodal iontophoresis compared to anodal iontophoresis. Both electroosmosis and electrophoresis affected iontophoretic fluxes of the macromolecules; the relative contributions of electroosmosis and electrophoresis were a function of molecular size and charge of the macromolecules.
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影响因子：
13.7
作者：
Michels, S;Rosenfeld, PJ;Venkatraman, AS
通讯作者：
Venkatraman, AS
影响因子：
2
作者：
Eljarrat-Binstock, Esther;Domb, Abraham J.;Pe'er, Jacob
通讯作者：
Pe'er, Jacob
影响因子：
2
作者：
Eljarrat-Binstock, Esther;Domb, Abraham J.;Pe'er, Jacob
通讯作者：
Pe'er, Jacob
影响因子：
3.4
作者：
Behar-Cohen, FF;El Aouni, A;Parel, JM
通讯作者：
Parel, JM
影响因子：
3.4
作者：
BeharCohen, FF;Parel, JM;DeKozak, Y
通讯作者：
DeKozak, Y