Interleukin 12: A Potential Link between Nerve Cells and the Immune Response in Inflammatory Disorders
Interleukin 12: A Potential Link between Nerve Cells and the Immune Response in Inflammatory Disorders
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白细胞介素 12：神经细胞与炎症性疾病免疫反应之间的潜在联系
作者：
Laurence A. Turka;Richard E. Goodman;J. Lynn Rutkowski;Anders A. F. Sima;A. Merry;Rajorshi Mitra;T. Wrone;G. Toews;Robert M. Strieter;B. Nickoloff
背景神经系统与一些炎症性皮肤病有关，这些证据包括应激在诱发病变中的作用、病变的对称性和失神经皮肤的保留。白细胞介素12（IL-12）是一种细胞因子，最近被证明可以促进细胞免疫反应，其特征在于迟发型超敏反应和TH 1-淋巴因子，干扰素-γ的生产。Materials and MethodsUsing免疫组织化学，IL-12免疫反应性被确定在正常和患病的人皮肤样本的冷冻切片，并在啮齿动物和人体组织样本的外周和中枢神经系统。采用逆转录-聚合酶链反应检测组织样本和培养细胞中IL-12 p35和p40 mRNA。IL-12蛋白水平也进行了检查，ELISA和定量生物测定利用IL-12依赖的细胞line.Resultsby免疫染色IL-12检测在正常和患病的皮肤样品的表皮中的游离神经末梢，也在真皮神经纤维。在轴突过程和各种胶质细胞类型中检测到强反应性。此外，IL-12的蛋白和mRNA中所含的皮肤周围神经和脊髓组织，和IL-12的功能水平产生的培养的雪旺细胞。ConclusionsIt可能是IL-12是重要的启动或繁殖选定的炎症性皮肤病变，并在确定的疾病模式，将发展。神经组织中IL-12的存在表明神经系统可以改变或放大皮肤和其他免疫反应的机制。
BackgroundThe nervous system has been implicated in several inflammatory skin disorders based on evidence such as the role of stress in inducing lesions, symmetry of lesions, and sparing of denervated skin. Interleukin 12 (IL-12) is a cytokine recently shown to promote cellular immune responses characterized by delayed-type hypersensitivity and production of the TH1-lymphokine, interferon-γ.Materials and MethodsUsing immunohistochemistry, IL-12 immunoreactivity was identified in cryostat sections of normal and diseased human skin samples, and in the peripheral and central nervous system of rodents and human tissue samples. IL-12 p35 and p40 mRNAs were detected using reverse transcriptase-polymerase chain reaction in tissue samples and cultured cells. IL-12 protein levels were also examined by ELISA and quantitative bioassay utilizing an IL-12-dependent cell line.ResultsBy immunostaining IL-12 was detected in free nerve ending in the epidermis of normal and diseased skin samples, and also in the dermal nerve fibers. Strong reactivity was detected in axonal processes and in various glial cell types. In addition, IL-12 protein and mRNA were contained within cutaneous peripheral nerves and spinal cord tissues, and functional levels of IL-12 were produced by cultured Schwann cells.ConclusionsIt is likely that IL-12 is important in initiating or propagating selected inflammatory skin lesions and in determining the pattern of disease that will develop. The presence of IL-12 in neural tissue suggests a mechanism whereby the nervous system can modify or amplify cutaneous and perhaps other immune responses.
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来自培养的 OX8-OX22-胸导管 T 细胞 RNA 的大鼠白细胞介素 10 (IL-10) cDNA 克隆的合成和表征。
DOI：
10.1016/0006-291x(92)91516-s
发表时间：
1992
影响因子：
3.1
作者：
Goodman,RE;Oblak,J;Bell,RG
通讯作者：
Bell,RG
角质形成细胞衍生的 T 细胞共刺激会优先产生 IL-2 和 IL-4，但不会优先产生 IFN-γ。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Goodman,RE;Nestle,F;Naidu,YM;Green,JM;Thompson,CB;Nickoloff,BJ;Turka,LA
通讯作者：
Turka,LA
影响因子：
56.9
作者：
STAHL, N;BOULTON, TG;YANCOPOULOS, GD
通讯作者：
YANCOPOULOS, GD
影响因子：
4.4
作者：
PA Sieling;X. Wang;MK Gately;JL Oliveros;T. McHugh;PF Barnes;Sf Wolf;L. Golkar;M. Yamamura;Y. Yogi;K. Uyemura;TH Rea;RL Modlin
通讯作者：
PA Sieling;X. Wang;MK Gately;JL Oliveros;T. McHugh;PF Barnes;Sf Wolf;L. Golkar;M. Yamamura;Y. Yogi;K. Uyemura;TH Rea;RL Modlin
影响因子：
6.5
作者：
UYEMURA, K;YAMAMURA, M;NICKOLOFF, BJ
通讯作者：
NICKOLOFF, BJ