Migration and differentiation of neural progenitor cells after recurrent laryngeal nerve avulsion in rats.
Migration and differentiation of neural progenitor cells after recurrent laryngeal nerve avulsion in rats.
复制标题
大鼠喉返神经撕脱后神经祖细胞的迁移和分化
DOI：
10.1371/journal.pone.0107288
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Zhao W;Xu W
目的探讨喉返神经撕脱伤后神经祖细胞（NPCs）从室管膜层向模糊性核（NA）的迁移和分化情况。所有动物均在左侧脑室注射CM-DiI。选取45只成年大鼠进行左RLN撕脱伤，9只大鼠作为对照。5-溴-2-脱氧尿苷（BrdU）腹腔注射。在RLN损伤后不同时间点对脑干进行免疫组化分析。RLN撕脱后，室管膜层的CM-DiI+ npc向受损NA迁移。迁移流中观察到CM-DiI+/GFAP+星形细胞、CM-DiI+/DCX+神经母细胞和CM-DiI+/NeuN+神经元。然而，同侧NA仅包括CM-DiI+星形胶质细胞，未包括新生神经元。RLN撕脱后，第四脑室室管膜层或中央管内的NPCs试图修复受损的NA。我们首先确认迁移流包括神经元和神经胶质细胞分化。然而，只有已分化的星形胶质细胞被成功纳入NA。这两种细胞类型在迁移过程中的存在可能在RLN损伤的修复中发挥作用。
To investigate migration and differentiation of neural progenitor cells (NPCs) from the ependymal layer to the nucleus ambiguus (NA) after recurrent laryngeal nerve (RLN) avulsion. All of the animals received a CM-DiI injection in the left lateral ventricle. Forty-five adult rats were subjected to a left RLN avulsion injury, and nine rats were used as controls. 5-Bromo-2-deoxyuridine (BrdU) was injected intraperitoneally. Immunohistochemical analyses were performed in the brain stems at different time points after RLN injury. After RLN avulsion, the CM-DiI+ NPCs from the ependymal layer migrated to the lesioned NA. CM-DiI+/GFAP+ astrocytes, CM-DiI+/DCX+ neuroblasts and CM-DiI+/NeuN+ neurons were observed in the migratory stream. However, the ipsilateral NA included only CM-DiI+ astrocytes, not newborn neurons. After RLN avulsion, the NPCs in the ependymal layer of the 4th ventricle or central canal attempt to restore the damaged NA. We first confirm that the migratory stream includes both neurons and glia differentiated from the NPCs. However, only differentiated astrocytes are successfully incorporated into the NA. The presence of both cell types in the migratory process may play a role in repairing RLN injuries.
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影响因子：
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