Effect of turmeric and curcumin on BP-DNA adducts.
Effect of turmeric and curcumin on BP-DNA adducts.
复制标题
姜黄和姜黄素对 BP-DNA 加合物的影响。
作者：
M. Mukundan;M. Chacko;V. Annapurna;K. Krishnaswamy
今天广泛流行的许多人类癌症可以通过改变生活方式来预防，饮食似乎是其中的一个重要因素。有几种饮食成分可以调节致癌过程，防止遗传毒性。包括姜黄在内的许多植物成分似乎都是有效的抗诱变剂和抗氧化剂。因此，我们用新发展的~(32)P-后标记方法研究了姜黄素和姜黄素对苯并[a]芘诱导的大鼠肝脏DNA加合物水平的调节作用。姜黄的活性成分(姜黄素)在饲料中添加0.1%、0.5%和3%时显著降低BP-DNA加合物(包括主要加合物DG-N_2-BP)的水平，而姜黄素的活性成分姜黄素在饲料中的添加水平为0.03%时显著降低24小时内形成的BP-DNA加合物的水平。注射苯并[a]芘。根据姜黄潜在的抗癌作用，讨论了这些作用的意义。此外，这些结果加强了姜黄的各种其他生物效应，这些效应与抗癌和化学预防直接相关。
Many human cancers that are widely prevalent today can be prevented through modifications in life-styles, of which diet appears to be an important agent. Several dietary constituents modulate the process of carcinogenesis and prevent genotoxicity. Many plant constituents including turmeric appear to be potent antimutagens and antioxidants. Therefore the modulatory effects of turmeric and curcumin on the levels of benzo[a]pyrene induced DNA adducts in the livers of rats were studied by the newly developed 32P-postlabelling assay method. Turmeric when fed at 0.1, 0.5 and 3% and the active principle of turmeric (curcumin) when fed at a level of 0.03% in the diet for 4 weeks significantly reduced the level of BP-DNA adducts including the major adduct dG-N2-BP, formed within 24 h in response to a single i.p. injection of benzo[a]pyrene. The significance of these effects in terms of the potential anticarcinogenic effects of turmeric is discussed. Further, these results strengthen the various other biological effects of turmeric which have direct relevance to anticarcinogenesis and chemoprevention.
登录
查看更多内容
影响因子：
8.9
作者：
Fiala,ES;Reddy,BS;Weisburger,JH
通讯作者：
Weisburger,JH
暴露于 7β,8α-二羟基-9α,10α-环氧-7,8,9,10-四氢苯并(a)芘的纯光学对映异构体的小鼠皮肤中 DNA、RNA 和蛋白质加合物的形成和持久性
影响因子：
11.2
作者：
Pelling,JC;Slaga,TJ;DiGiovanni,J
通讯作者：
DiGiovanni,J
影响因子：
56.9
作者：
EVERSON, RB;RANDERATH, E;RANDERATH, K
通讯作者：
RANDERATH, K
影响因子：
4.7
作者：
REDDY, MV;GUPTA, RC;RANDERATH, K
通讯作者：
RANDERATH, K
32P-加合物测定：不同增强程序中结构多样的 DNA 加合物的比较回收率。
影响因子：
4.7
作者：
Gupta,RC;Earley,K
通讯作者：
Earley,K