Establishment of a murine breast tumor model by subcutaneous or orthotopic implantation.
Establishment of a murine breast tumor model by subcutaneous or orthotopic implantation.
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皮下或原位植入小鼠乳腺肿瘤模型的建立
DOI：
10.3892/ol.2018.8113
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发表时间：
2018-05
期刊：
影响因子：
2.9
通讯作者：
Wang XM
中科院分区：
文献类型：
--
作者：
Zhang Y;Zhang GL;Sun X;Cao KX;Ma C;Nan N;Yang GW;Yu MW;Wang XM
许多鼠模型用于模拟乳腺癌的病理学。使用原位植入（OS）和皮下植入（SQ）的组织接种和细胞接种通常用于产生小鼠模型以研究癌症。然而，关于这些方法的变化，现有资料有限。本研究比较了使用OS和SQ产生的肿瘤的生长、转移、存活和组织病理学，以表征由两种不同方法产生的肿瘤的特征。此外，本研究旨在为研究人员设计实验时提供更多的选择。使用OS或SQ将4 T1-luc 2细胞悬液或4 T1-luc 2组织悬液接种到BALB/c小鼠中。使用体内成像系统和卡尺检测肿瘤生长和转移。切除的肿瘤和肺通过用苏木精和伊红以及分化的血管标记物簇31进行组织染色来评估。本研究的结果表明，与组织悬浮液产生的肿瘤相比，细胞悬浮液产生的乳腺肿瘤尺寸增加，接种后使用卡尺在更早的时间点进行可视化和确定。与SQ肿瘤相比，OS肿瘤的生物发光增加趋势更明显。与SQ肿瘤相比，OS肿瘤体积增大，变异减小。此外，OS肿瘤表现出微血管密度增加。关于转移的生物发光信号和组织学结果是一致的：OS植入与SQ植入相比产生了增加的肺转移，尽管它们表现出相似的存活时间。本研究的结果表明，来自不同来源（组织或细胞）的接种物影响肿瘤生长。此外，乳腺肿瘤的进展和组织病理学特征在OS和SQ之间是不同的，而OS表现出恶性行为增加。了解不同方法建立的小鼠乳腺癌模型的特点，有助于研究者根据研究需要选择合适的动物模型。
A number of murine models are used to mimic the pathology of breast cancer. Tissue inoculation and cell inoculation using orthotopic implantation (OS) and subcutaneous implantation (SQ) are commonly used to generate murine models to investigate cancer. However, limited information is available in regard to the variations of these methods. The present study compared growth, metastasis, survival and histopathology of tumors produced using OS and SQ to characterize features of the tumors produced by the two distinct methods. Additionally, the present study aimed at providing increased options for investigators when designing experiments. 4T1-luc2 cell suspension or 4T1-luc2 tissue suspension was inoculated using either OS or SQ into BALB/c mice. Tumor growth and metastasis were detected using an in vivo imaging system and calipers. Excised tumors and lung were assessed by tissue staining with hematoxylin and eosin, and the vessel marker cluster of differentiation 31. The results of the present study revealed that the cell suspension generated breast tumors of increased size, which was visualized and determined, following inoculation, using calipers at an earlier time point compared with tumors produced by tissue suspension. The increasing bioluminescent trend of OS tumors was more marked compared with that of SQ tumors. The volume of OS tumor was increased with decreased variation, compared with that of SQ tumors. In addition, the OS tumor exhibited increased microvessel density. Bioluminescent signals and histological results in regard to metastasis were consistent: OS implantation produced increased lung metastasis compared with that of SQ implantation, although they exhibited similar survival times. The results of the present study indicated that the inocula from distinct sources (tissue or cell) affected tumor growth. Furthermore, breast tumor progression and histopathological characteristics were distinct between OS and SQ, whereas OS exhibited increased malignant behavior. Understanding the characteristics of murine breast cancer models established by diverse methods may aid investigators to select appropriate animal models, according to the requirements of the study.
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影响因子：
--
作者：
Menezes, Mitchell E.;Das, Swadesh K.;Emdad, Luni;Windle, Jolene J.;Wang, Xiang-Yang;Sarkar, Devanand;Fisher, Paul B.
通讯作者：
Fisher, Paul B.
影响因子：
3.7
作者：
Lau C;Zhou IY;Cheung MM;Chan KC;Wu EX
通讯作者：
Wu EX
影响因子：
3.2
作者：
Gravekamp, C;Sypniewska, R;Reddick, R
通讯作者：
Reddick, R
影响因子：
3.7
作者：
Hammond, Edward;Brandt, Ralf;Dredge, Keith
通讯作者：
Dredge, Keith
影响因子：
9.7
作者：
Light, Donald W.
通讯作者：
Light, Donald W.