Altered lipid and salt taste responsivity in ghrelin and GOAT null mice.
Altered lipid and salt taste responsivity in ghrelin and GOAT null mice.
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脂质和盐的味道反应性改变了生长素和山羊无效的小鼠。
DOI：
10.1371/journal.pone.0076553
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Maudsley S
中科院分区：
文献类型：
--
作者：
Cai H;Cong WN;Daimon CM;Wang R;Tschöp MH;Sévigny J;Martin B;Maudsley S
味觉在调节食物偏好、进食行为和能量平衡方面起着重要作用。味觉受多种因素的调节，包括胃激素如生长激素释放肽。Ghrelin可以调节生长激素释放、食物摄入、肥胖和能量代谢。Ghrelin O-酰基转移酶（GOAT）对Ghrelin的辛酰化是一种特异性的翻译后修饰，对Ghrelin的许多生物学活性至关重要。Ghrelin和GOAT都在包括味觉系统在内的许多器官中广泛表达。在本研究中，在野生型（WT）、ghrelin敲除（ghrelin−/−）和GOAT敲除（GOAT−/−）小鼠中评估了总体代谢特征。与WT小鼠相比，Ghrelin−/−小鼠表现出摄食量减少、血浆甘油三酯增加和酮体增加，同时表现出WT样体重、脂肪组成和葡萄糖控制。相比之下，与WT小鼠相比，GOAT-/-小鼠的体重、肥胖、静息葡萄糖和胰岛素水平均降低。进行了简短的味觉行为测试，以确定WT、ghrelin−/−和GOAT−/−小鼠的味觉反应。Ghrelin和GOAT缺失小鼠具有降低的脂质味觉反应性。此外，我们发现，与WT小鼠相比，ghrelin−/−小鼠的咸味反应减弱，但GOAT−/−小鼠的咸味反应增强。潜在的脂质味觉调节剂Cd 36和Gpr 120的表达在ghrelin和GOAT null小鼠的味蕾中减少，而盐敏感的ENaC亚基在GOAT−/−小鼠中与WT小鼠相比增加。Cd 36、Gpr 120和ENaC的表达改变可能是ghrelin−/−和GOAT−/−小鼠脂质和盐味感知改变的原因。目前研究中的数据可能暗示了ghrelin信号传导活性在脂质和盐味觉模式的调节中。
Taste perception plays an important role in regulating food preference, eating behavior and energy homeostasis. Taste perception is modulated by a variety of factors, including gastric hormones such as ghrelin. Ghrelin can regulate growth hormone release, food intake, adiposity, and energy metabolism. Octanoylation of ghrelin by ghrelin O-acyltransferase (GOAT) is a specific post-translational modification which is essential for many biological activities of ghrelin. Ghrelin and GOAT are both widely expressed in many organs including the gustatory system. In the current study, overall metabolic profiles were assessed in wild-type (WT), ghrelin knockout (ghrelin−/−), and GOAT knockout (GOAT−/−) mice. Ghrelin−/− mice exhibited decreased food intake, increased plasma triglycerides and increased ketone bodies compared to WT mice while demonstrating WT-like body weight, fat composition and glucose control. In contrast GOAT−/− mice exhibited reduced body weight, adiposity, resting glucose and insulin levels compared to WT mice. Brief access taste behavioral tests were performed to determine taste responsivity in WT, ghrelin−/− and GOAT−/− mice. Ghrelin and GOAT null mice possessed reduced lipid taste responsivity. Furthermore, we found that salty taste responsivity was attenuated in ghrelin−/− mice, yet potentiated in GOAT−/− mice compared to WT mice. Expression of the potential lipid taste regulators Cd36 and Gpr120 were reduced in the taste buds of ghrelin and GOAT null mice, while the salt-sensitive ENaC subunit was increased in GOAT−/− mice compared with WT mice. The altered expression of Cd36, Gpr120 and ENaC may be responsible for the altered lipid and salt taste perception in ghrelin−/− and GOAT−/− mice. The data presented in the current study potentially implicates ghrelin signaling activity in the modulation of both lipid and salt taste modalities.
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味觉的细胞生物学。
影响因子：
7.8
作者：
Chaudhari, Nirupa;Roper, Stephen D.
通讯作者：
Roper, Stephen D.
营养状况改变大鼠味蕾中糖精摄入量和甜味受体 mRNA 表达
影响因子：
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作者：
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DOI：
10.1159/000093760
发表时间：
2006-01-01
期刊：
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影响因子：
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作者：
Breslin, Paul A. S.;Huang, Liquan
通讯作者：
Huang, Liquan
影响因子：
16.2
作者：
Cowley, MA;Smith, RG;Horvath, TL
通讯作者：
Horvath, TL
影响因子：
3.7
作者：
Cong WN;Cai H;Wang R;Daimon CM;Maudsley S;Raber K;Canneva F;von Hörsten S;Martin B
通讯作者：
Martin B