High affinity binding sites for somatostatin to rat pituitary.
High affinity binding sites for somatostatin to rat pituitary.
复制标题
生长抑素与大鼠垂体的高亲和力结合位点。
DOI：
10.1016/0006-291x(82)90963-9
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
W. Vale
中科院分区：
文献类型：
--
作者：
J. Reubi;M. Perrin;J. Rivier;W. Vale
用[125I-Tyr11]-SS-14或[Leu8， d - trp22125i - tyr25]-SS-28作为放射性配体描述了大鼠垂体膜中生长抑素（SS）的结合位点；在每一种情况下，SS的KD值分别为1.09 nM和0.95 nM的饱和和高亲和力结合位点已被表征。结合能力为100 f mol /mg蛋白质。放射性受体测定中测定的各种SS类似物的效力与测定生长激素释放抑制的生物测定中的效力一致；特别是，SS-28的威力略低于SS-14。将这些数据与SS在脑和胰腺结合的数据进行比较，表明SS在垂体、脑和胰腺的结合位点可能存在一些药理学差异。
Binding sites for somatostatin (SS) are described in rat pituitary membranes using either [125I-Tyr11]-SS-14 or [Leu8, D-Trp22,125I-Tyr25]-SS-28 as radioligands; in each case saturable and high affinity binding sites with KD's for SS of 1.09 and 0.95 nM respectively have been characterized. The binding capacity is 100 f mols/mg protein. The potencies of various SS analogs measured in the radioreceptor assay are in agreement with the potencies in a bioassay measuring inhibition of growth hormone release; in particular, SS-28 is slightly less potent than SS-14. A comparison of these data with those describing SS binding in brain and pancreas suggests that some pharmacological differences may exist between pituitary, brain and pancreas binding sites for SS.
DOI：
10.1016/0006-291x(80)91415-1
发表时间：
1980-01-01
影响因子：
3.1
作者：
BOHLEN, P;BRAZEAU, P;GUILLEMIN, R
通讯作者：
GUILLEMIN, R
影响因子：
2.9
作者：
SPIESS, J;VILLARREAL, J;VALE, W
通讯作者：
VALE, W
胰腺 β 细胞上生长抑素 28 的特异性高亲和力结合位点：与脑生长抑素受体的差异。
影响因子：
4.8
作者：
Reubi,JC;Rivier,J;Perrin,M;Brown,M;Vale,W
通讯作者：
Vale,W