The cohesin stabilizer Sororin drives G(2)-M transition and spindle assembly in mammalian oocytes.
The cohesin stabilizer Sororin drives G(2)-M transition and spindle assembly in mammalian oocytes.
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粘连蛋白稳定剂 Sororin 驱动哺乳动物卵母细胞中的 G2-M 转变和纺锤体组装
DOI：
10.1126/sciadv.abg9335
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发表时间：
2021-09-24
期刊：
影响因子：
13.6
通讯作者：
Xiong B
中科院分区：
文献类型：
--
作者：
Zhou C;Zhang X;Miao Y;Zhang Y;Li Y;Xiong B
Sororin通过哺乳动物卵母细胞减数分裂I在驱动减数分裂恢复和进展中起非经典作用。在有丝分裂的S期，Sororin被乙酰化Smc 3募集，并通过抵消Wapl-Pds 5相互作用来稳定姐妹染色单体凝聚力。此后，Sororin在前期被磷酸化并转移到细胞质，在那里其功能仍然知之甚少。在这里，我们报告Sororin作为哺乳动物卵母细胞减数分裂G2-M转换和纺锤体组装的调节剂。Sororin存在于GV卵母细胞的核中，并在小鼠减数分裂I期间与纺锤体结合。Sororin的耗尽会导致GVBD失败，原因是Cdk 1失活以及细胞周期蛋白B2水平降低导致纺锤体组装缺陷。我们验证Sororin与细胞周期蛋白B2的相互作用，保护其免受APCCdh 1的破坏，APCCdh 1驱动M相进入和双极纺锤体形成。值得注意的是，Sororin的减数分裂功能在哺乳动物中是保守的。总之，我们的研究结果提供了新的见解Sororin在哺乳动物卵母细胞减数分裂I的恢复和进展的非经典作用。
Sororin plays a noncanonical role in driving meiotic resumption and progression through meiosis I in mammalian oocytes. During the S phase of mitosis, Sororin is recruited by acetylated Smc3 and stabilizes sister chromatid cohesion by counteracting the Wapl-Pds5 interaction. Thereafter, Sororin is phosphorylated during prophase and translocated to the cytoplasm, where its function remains poorly understood. Here, we report that Sororin acts as a regulator of meiotic G2-M transition and spindle assembly in mammalian oocytes. Sororin is present in the nucleus of GV oocytes and becomes associated with the spindle apparatus during meiosis I in mice. Depletion of Sororin causes failure of GVBD due to inactivation of Cdk1 and defective spindle assembly because of reduced levels of Cyclin B2. We validate Sororin interactions with Cyclin B2 that protects it from destruction by APCCdh1, which drives M phase entry and bipolar spindle formation. Notably, the meiotic functions of Sororin are conserved among mammals. Together, our findings provide novel insights into the noncanonical role of Sororin in the resumption of meiosis and progression through meiosis I in mammalian oocytes.
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内聚建立因子 Esco1 乙酰化 α-微管蛋白，以确保卵母细胞减数分裂中纺锤体的正确组装。
影响因子：
14.9
作者：
Lu Y;Li S;Cui Z;Dai X;Zhang M;Miao Y;Zhou C;Ou X;Xiong B
通讯作者：
Xiong B
影响因子：
11.8
作者：
Gui, Liming;Homer, Hayden
通讯作者：
Homer, Hayden
DOI：
10.1083/jcb.153.1.137
发表时间：
2001-04-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Geley S;Kramer E;Gieffers C;Gannon J;Peters JM;Hunt T
通讯作者：
Hunt T
影响因子：
16
作者：
Rankin, S;Ayad, NG;Kirschner, MW
通讯作者：
Kirschner, MW
影响因子：
9.2
作者：
Giménez-Abián, JF;Sumara, I;Peters, JM
通讯作者：
Peters, JM