Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
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DOI：
10.1083/jcb.153.1.137
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发表时间：
2001-04-02
期刊：
影响因子：
--
通讯作者：
Hunt T
中科院分区：
文献类型：
--
作者：
Geley S;Kramer E;Gieffers C;Gannon J;Peters JM;Hunt T
细胞周期蛋白A在S期和G2期是稳定的蛋白质，但当细胞进入有丝分裂时不稳定，并且在中期到后期转变之前几乎完全降解。显微注射针对后期促进复合物/细胞周期体（APC/C）或针对人Cdc 20（泡沫）的亚基的抗体在中期阻滞细胞并稳定细胞周期蛋白A和B1。细胞周期蛋白A在体外被Cdc 20或Cdh 1激活的APC/C有效地多泛素化，但与细胞周期蛋白B1相反，细胞周期蛋白A的蛋白水解没有被纺锤体组装检查点延迟。抑制纺锤体组装检查点可加速细胞周期蛋白B1的降解。这些数据表明，APC/C被激活细胞进入有丝分裂，并立即目标细胞周期蛋白A的降解，而纺锤体组装检查点延迟细胞周期蛋白B1的降解，直到中期到后期的过渡。细胞周期蛋白A的“破坏盒”（D-box）比细胞周期蛋白B长10-20个残基。野生型细胞周期蛋白A的过表达延迟了中期到后期的过渡，而缺乏D-box的细胞周期蛋白A突变体的表达在后期逮捕细胞。
Cyclin A is a stable protein in S and G2 phases, but is destabilized when cells enter mitosis and is almost completely degraded before the metaphase to anaphase transition. Microinjection of antibodies against subunits of the anaphase-promoting complex/cyclosome (APC/C) or against human Cdc20 (fizzy) arrested cells at metaphase and stabilized both cyclins A and B1. Cyclin A was efficiently polyubiquitylated by Cdc20 or Cdh1-activated APC/C in vitro, but in contrast to cyclin B1, the proteolysis of cyclin A was not delayed by the spindle assembly checkpoint. The degradation of cyclin B1 was accelerated by inhibition of the spindle assembly checkpoint. These data suggest that the APC/C is activated as cells enter mitosis and immediately targets cyclin A for degradation, whereas the spindle assembly checkpoint delays the degradation of cyclin B1 until the metaphase to anaphase transition. The “destruction box” (D-box) of cyclin A is 10–20 residues longer than that of cyclin B. Overexpression of wild-type cyclin A delayed the metaphase to anaphase transition, whereas expression of cyclin A mutants lacking a D-box arrested cells in anaphase.
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DOI：
10.1083/jcb.116.3.707
发表时间：
1992-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hunt T;Luca FC;Ruderman JV
通讯作者：
Ruderman JV
影响因子：
10.5
作者：
CohenFix, O;Peters, JM;Koshland, D
通讯作者：
Koshland, D
影响因子：
64.5
作者：
KING, RW;PETERS, JM;KIRSCHNER, MW
通讯作者：
KIRSCHNER, MW
影响因子：
64.5
作者：
Jin, DY;Spencer, F;Jeang, KT
通讯作者：
Jeang, KT
影响因子：
11.4
作者：
Klotzbucher, A;Stewart, E;Hunt, T
通讯作者：
Hunt, T