Analysis of the temporal requirement for eda in hair and sweat gland development.
Analysis of the temporal requirement for eda in hair and sweat gland development.
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分析头发和汗腺发育中EDA的时间需求。
DOI：
10.1038/jid.2008.318
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发表时间：
2009-04
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EDA信号传导在皮肤附属器起始中起关键作用。然而，其可能参与的附属物亚型的确定和诱导后阶段的附属物的发展，还没有系统地研究。为了解决这些问题，我们在四环素调节的条件性小鼠模型中操纵Eda-A1转基因表达，其中转基因是活性外胚层发育不良（Eda）的唯一来源。我们发现，Eda-A1恢复汗腺和所有的头发亚型在虎斑猫，但每个都需要它的行动在一个特殊的发展时间：由E17后卫，由E19锥，并开始在E18锯齿/auchen头发。保卫和锥毛与野生型的毛没有区别，但恢复的锯齿形和奥肯毛，虽然可识别，有点小，缺乏特征弯曲。值得注意的是，次级毛囊形成的锥，auchen，和锯齿形的头发需要更高的Eda-A1表达水平比后卫毛或汗腺。此外，Eda-A1的表达是需要的，直到早期的毛乳头阶段的保卫毛胚，使毛囊，但其成熟是不必要的。类似地，汗腺栓需要Eda-A1在早期形成成熟的腺体。因此，我们推断，EDA信号是需要在时空限制的时间间隔的各种皮肤附件的确定和发展。
EDA signaling plays a pivotal role in skin appendage initiation. Its possible involvement in appendage subtype determination and post-induction stage appendage development, however, has not been studied systematically. To address these issues we manipulated Eda-A1 transgene expression in a tetracycline-regulated conditional mouse model, where the transgene is the only source of active ectodysplasin (Eda). We find that Eda-A1 restores sweat glands and all hair subtypes in Tabby, but each requires its action at an idiosyncratic time of development: by E17 for guard, by E19 for awl, and starting at E18 for zigzag/auchen hair. Guard and awl hairs were indistinguishable from their wild-type counterparts; but restored zigzag and auchen hairs, while recognizable, were somewhat smaller and lacked characteristic bends. Notably, secondary hair follicle formation of awl, auchen, and zigzag hairs required higher Eda-A1 expression level than did guard hair or sweat glands. Furthermore, Eda-A1 expression is required until the early dermal papilla stage for guard hair germs to make follicles, but is dispensable for their maturation. Similarly, sweat gland pegs require Eda-A1 at an early stage to form mature glands. Thus we infer that EDA signaling is needed for the determination and development of various skin appendages at spatiotemporally restricted intervals.
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